Fluorescence detection method for organophosphorus pesticide residues

Abstract

The invention discloses a fluorescence detection method for organophosphorus pesticide residues, and belongs to the technical field of detection, and the method comprises the following steps: 1), pretreatment of a sample to be tested, to be more specific, washing the sample to be tested, peeling the sample to be tested, cutting into pieces, adding anhydrous methanol, performing ultrasonic treatment, filtering and diluting to obtain filtrate reserve liquid for standby use; 2), drawing of a standard curve, to be more specific, taking dimethoate solutions with different concentrations, respectively adding a Tris-HCl buffer solution, a urease solution, a urea solution and a pyrogallic acid solution for reaction, and adding polymer quantum dots; and measuring fluorescence value of each system,making a standard curve, and calculating the regression equation of the standard curve; and 3) determination of the sample, to be more specific, taking the filtrate reserve liquid obtained in the step1), respectively adding a Tris-HCl buffer solution, a urease reserve solution, a urea reserve solution and a pyrogallic acid solution for reaction, and adding the polymer quantum dots; and measuringfluorescence value of the sample, and substituting the measured fluorescence value into the regression equation of the step 2 to obtain the residue amount of the dimethoate solution in the sample.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a fluorescence detection method for organophosphorus pesticide residues. Background technique [0002] As a large agricultural country, my country's agriculture plays a decisive role in the development of the national economy and people's lives. Chemical pesticides are indispensable and important means of production in my country's agricultural production due to their advantages such as wide inhibition range, fast action effect, good stability, and easy storage and transportation. Organophosphorus pesticides are a kind of organophosphate compounds. Due to their wide range of uses, various types, easy decomposition, fast drug effect, and difficult accumulation, they have become the most widely used insecticides. Pesticide residues are an inevitable phenomenon after the application of pesticides. Pesticides remain in the atmosphere, fruits and vegetables, soil, and w...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the increasingly serious problem of organophosphorus pesticide residues in the prior art, which not only causes environmental pollution, but also causes harm to human health after food enters the human body, the purpose of the present invention is to provide a method with high accuracy and reproducibility. A good, high-sensitivity, low-cost fluorescence detection method for organophosphorus pesticide residues, mainly using semiconductor polymer quantum dots as fluorescent probes, constructs a quantum dot detection system based on enzyme inhibition method, and provides a basis for the detection of organophosphorus pesticide residues. new method

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