Primers, probe and kit for detecting mutation of C-KIT gene at site 1689 to 1718

Abstract

The invention discloses primers, a probe and a kit for detecting mutation of a C-KIT gene at site 1689 to 1718. The primers are as shown in SEQ ID No. 1 and SEQ ID No. 2, and the probe is as shown inSEQ ID No. 3. When the kit provided by the invention is applied to real-time fluorescent PCR, the substitution mutation of GGGTCT at the site 1689 to 1718 of the C-KIT gene can be detected.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer, a probe and a related kit for detecting C-KIT gene mutation. Background technique [0002] C-KIT is a proto-oncogene, located on chromosome 4q11-12, with a total length of about 80kb. The C-KIT protein encoded by C-KIT belongs to the type III receptor tyrosine kinase family member. It is a transmembrane protein with a molecular weight of about 145KD, including the intracellular tyrosine kinase domain, transmembrane domain and ligand binding Site extracellular region. When the ligand binds to it, it can activate its own tyrosine protein kinase activity, and activate the downstream signal transduction pathway through a series of reactions, thereby regulating the growth and proliferation of cells. [0003] Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the digestive tract. Studies have shown that the C-KIT gene mutation rate is about 90% in GI...

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Problems solved by technology

However, the low sensitivity of this method will lead to missed detection and false negative, and the detection time is long, which cannot meet the actual needs of clinical detection

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