The invention discloses a primer combination for detecting
mutation of a C-
KIT gene in trace tissue. The primer combination comprises a pre-amplification primer group, a primer group for detecting No.9
exon mutation, a primer group for detecting No.11
exon mutation, a primer group for detecting No.13
exon mutation, a primer group for detecting No.14 exon mutation, a primer group for detecting No.17 exon mutation and a primer group for detecting No.18 exon mutation. According to a method, a trace amount of
DNA is pre-amplified to obtain a high-concentration
DNA template, and the mutation condition of the C-
KIT gene in a sample is detected by combining a
direct sequencing method; by applying the primer combination to preparation of a detection
reagent for detecting mutation of the C-
KIT gene, the
DNA concentration of the detectable sample is low up to 100 pg, and all mutation of a No.9 exon, a No.11 exon, a No.13 exon, a No.14 exon, a No.17 exon and a No.18 exon of the C-KIT
gene can be simultaneously detected. The detection method is high in sensitivity and specificity, low in cost and suitable for C-KIT
gene mutation detection on a clinical
tumor patient and has the very good clinical application value.