Primer, probe and kit for detection of C-KIT gene mutation

Abstract

The invention discloses a primer, a probe and a kit for detection of C-KIT gene mutation. The primer is represented as the SEQ ID No.1 and the SEQ ID No.2 and the probe is represented as the SEQ ID No.3. By means of the kit for real-time fluorescent PCR, T-replacement mutation on the 1700-1733 loci of C-KIT gene can be detected.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer, a probe and a related kit for detecting C-KIT gene mutation. Background technique [0002] C-KIT is a proto-oncogene, located on chromosome 4q11-12, with a total length of about 80kb. The C-KIT protein encoded by C-KIT belongs to the type III receptor tyrosine kinase family member. It is a transmembrane protein with a molecular weight of about 145KD, including the intracellular tyrosine kinase domain, transmembrane domain and ligand binding Site extracellular region. When the ligand binds to it, it can activate its own tyrosine protein kinase activity, and activate the downstream signal transduction pathway through a series of reactions, thereby regulating the growth and proliferation of cells. [0003] Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the digestive tract. Studies have shown that the C-KIT gene mutation rate is about 90% in GI...

AI Technical Summary

Problems solved by technology

However, the low sensitivity of this method will lead to missed detection and false negative, and the detection time is long, which cannot meet the actual needs of clinical detection

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