Primer, probe and kit for detecting mutation of 1654 to 1689 sites of C-KIT gene
A kit and gene technology, applied in the biological field, can solve the problems of long detection time, low sensitivity, false negative, etc., and achieve the effect of fast detection speed, high sensitivity and strong specificity
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[0019] Below in conjunction with specific embodiment, the present invention is further elaborated. It should be understood that these examples are only for the present invention and are not intended to limit the scope of the present invention. For the experimental methods without specific conditions indicated in the examples, the conventional conditions well known to those skilled in the art, or the conditions suggested by the manufacturer were followed.
[0020] 1. Principle
[0021] After the specific primers and probes of the present invention are combined with the DNA template, Taq DNA polymerase uses deoxynucleotide (dNTP) as a substrate to carry out in vitro tests on the internal reference gene (Internal Reference, IR) and the C-KIT gene mutant gene. Amplify. Fluorescent PCR technology is used for detection, and fluorescence is released through hydrolysis of specific probes to monitor the progress of PCR reaction and determine the mutation of C-KIT gene.
[0022] The ...
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