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Primer, probe and kit for detecting mutation of 1654 to 1689 sites of C-KIT gene

A kit and gene technology, applied in the biological field, can solve the problems of long detection time, low sensitivity, false negative, etc., and achieve the effect of fast detection speed, high sensitivity and strong specificity

Inactive Publication Date: 2018-02-23
BEIJING ACCB BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the low sensitivity of this method will lead to missed detection and false negative, and the detection time is long, which cannot meet the actual needs of clinical detection

Method used

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  • Primer, probe and kit for detecting mutation of 1654 to 1689 sites of C-KIT gene
  • Primer, probe and kit for detecting mutation of 1654 to 1689 sites of C-KIT gene
  • Primer, probe and kit for detecting mutation of 1654 to 1689 sites of C-KIT gene

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Embodiment Construction

[0019] Below in conjunction with specific embodiment, the present invention is further elaborated. It should be understood that these examples are only for the present invention and are not intended to limit the scope of the present invention. For the experimental methods without specific conditions indicated in the examples, the conventional conditions well known to those skilled in the art, or the conditions suggested by the manufacturer were followed.

[0020] 1. Principle

[0021] After the specific primers and probes of the present invention are combined with the DNA template, Taq DNA polymerase uses deoxynucleotide (dNTP) as a substrate to carry out in vitro tests on the internal reference gene (Internal Reference, IR) and the C-KIT gene mutant gene. Amplify. Fluorescent PCR technology is used for detection, and fluorescence is released through hydrolysis of specific probes to monitor the progress of PCR reaction and determine the mutation of C-KIT gene.

[0022] The ...

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PUM

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Abstract

The invention discloses a primer, a probe and a kit for detecting mutation of 1654 to 1689 sites of a C-KIT gene. The primer is shown as SEQ ID No.1 to SEQ ID No.2; the probe is shown as SEQ ID No.3.When the kit disclosed by the invention is used for carrying out real-time fluorescence PCR (Polymerase Chain Reaction), deletion mutation of the 1654 to 1689 sites of the C-KIT gene can be detected.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer, a probe and a related kit for detecting C-KIT gene mutation. Background technique [0002] C-KIT is a proto-oncogene, located on chromosome 4q11-12, with a total length of about 80kb. The C-KIT protein encoded by C-KIT belongs to the type III receptor tyrosine kinase family member. It is a transmembrane protein with a molecular weight of about 145KD, including the intracellular tyrosine kinase domain, transmembrane domain and ligand binding Site extracellular region. When the ligand binds to it, it can activate its own tyrosine protein kinase activity, and activate the downstream signal transduction pathway through a series of reactions, thereby regulating the growth and proliferation of cells. [0003] Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the digestive tract. Studies have shown that the C-KIT gene mutation rate is about 90% in GI...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/156C12Q2600/166
Inventor 莫敏俐李晖陈钊丁凤
Owner BEIJING ACCB BIOTECH
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