Primer, probe and kit for detecting mutation of 1675 to 1681 sites of C-KIT gene

Abstract

The invention discloses a primer, a probe and a kit for detecting mutation of 1675 to 1681 sites of a C-KIT gene. The primer is shown as SEQ ID No.1 to SEQ ID No.2; the probe is shown as SEQ ID No.3.When the kit disclosed by the invention is used for carrying out real-time fluorescence PCR (Polymerase Chain Reaction), the 1675 to 1681 sites of the C-KIT gene can be replaced with mutation of A.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer, a probe and a related kit for detecting C-KIT gene mutation. Background technique [0002] C-KIT is a proto-oncogene, located on chromosome 4q11-12, with a total length of about 80kb. The C-KIT protein encoded by C-KIT belongs to the type III receptor tyrosine kinase family member. It is a transmembrane protein with a molecular weight of about 145KD, including the intracellular tyrosine kinase domain, transmembrane domain and ligand binding Site extracellular region. When the ligand binds to it, it can activate its own tyrosine protein kinase activity, and activate the downstream signal transduction pathway through a series of reactions, thereby regulating the growth and proliferation of cells. [0003] Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the digestive tract. Studies have shown that the C-KIT gene mutation rate is about 90% in GI...

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Problems solved by technology

However, the low sensitivity of this method will lead to missed detection and false negative, and the detection time is long, which cannot meet the actual needs of clinical detection

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