c-KIT gene mutation detection liquid-phase chip

A detection solution and chip technology, applied in the field of molecular biology, can solve the problems of easy contamination of samples, high false positive rate, high price, etc., and achieve the effects of strong scalability, simple steps, and improved sensitivity.
CN101984070AActive Publication Date: 2011-03-09SUREXAM BIO TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUREXAM BIO TECH
Publication Date
2011-03-09

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Abstract

The invention discloses a c-KIT gene mutation detection liquid-phase chip, which comprises tag sequence at 5' terminal and ASPE (Allele Specific Primer Extension) primers consisting of specific primers specific to mutational sites at 3' terminal. The specific primers consist of sequences shown in SEQ ID No.10 and SEQ ID No.11 specific to AY502-503dup mutational sites, sequences shown in SEQ ID No.12 and SEQ ID No.13 specific to V560G mutational site, sequences shown in SEQ ID No.14 and SEQ ID No.15 specific to T670I mutational site and / or sequences shown in SEQ ID No.16-SEQ ID No.18specific to D816H / V mutational site, the tag sequence which is selected from sequences shown in SEQ ID No.1-SEQ ID No.9, microballoons which have different color codings and are respectively enveloped with a specific anti-tag sequence and amplification primers. The coincidence ratio of the result of sequencing method with that of the c-KIT gene mutation detection liquid-phase chip reaches up to 100 percent. The c-KIT gene mutation detection liquid-phase chip can simultaneously detect a plurality of mutational sites with excellent signal to noise ratio.
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Description

technical field

[0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, in particular to a c-KIT gene mutation detection liquid phase chip. Background technique

[0002] Gastrointestinal stromal tumor (GIST) is a special tumor originating from the gastrointestinal tract. In recent years, with the in-depth research on the molecular pathology of GIST and the application of targeted drugs, GIST has attracted more and more attention. Surgical resection is still the only curative treatment for GIST, but the recurrence rate is extremely high. The molecular targeted drug imatinib mesylate (Gleevec) has achieved very good curative effect in the treatment of GIST and is the drug of choice for GIST patients today.

[0003] The mechanism of action of imatinib is to inhibit the tyrosine kinase of platelet-derived growth factor (PDGFR) receptor and c-KIT receptor, thereby inhibiting the cell behavior mediated by PDGFR and c-KIT, and then i...

Claims

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