Primer, probe and kit for detecting 1737-1738 site mutations of C-KIT genes

Abstract

The invention discloses a primer, probe and kit for detecting 1737-1738 site mutations of C-KIT genes. The primer is as shown in SEQ ID No. 1-SEQ ID No.2, and the probe is as shown in the SEQ ID No.3.By using the kit disclosed by the invention for performing real-time fluorescence PCR, 1737-1738 site insertion TATGAT mutations of the C-KIT genes can be detected.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer, a probe and a related kit for detecting C-KIT gene mutation. Background technique [0002] C-KIT is a proto-oncogene, located on chromosome 4q11-12, with a total length of about 80kb. The C-KIT protein encoded by C-KIT belongs to the type III receptor tyrosine kinase family member. It is a transmembrane protein with a molecular weight of about 145KD, including the intracellular tyrosine kinase domain, transmembrane domain and ligand binding Site extracellular region. When the ligand binds to it, it can activate its own tyrosine protein kinase activity, and activate the downstream signal transduction pathway through a series of reactions, thereby regulating the growth and proliferation of cells. [0003] Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the digestive tract. Studies have shown that the C-KIT gene mutation rate is about 90% in GI...

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Problems solved by technology

However, the low sensitivity of this method will lead to missed detection and false negative, and the detection time is long, which cannot meet the actual needs of clinical detection

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