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A kind of rapid propagation and growth technology of white and tissue culture seedlings

A tissue culture seedling and rapid propagation technology, which is applied in the field of rapid propagation and growth technology of bletilla seedlings, can solve the problems of high technical requirements for direct seeding, high price of bletilla and finished products, difficulty in germination and growth, etc., to achieve shortening The effect of tissue culture time and cycle, shortened planting cycle, and simple operation

Active Publication Date: 2020-07-24
张俊岳 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are mainly three methods of propagation: tuber propagation, direct seeding and tissue culture. The first type of tuber propagation is the main source of seedlings due to its low technical requirements, simple propagation method, short time and relatively strong seedlings. However, tuber propagation It directly leads to the lack of finished products in the current market, and a large amount of fresh white and cost are used for tuber propagation instead of entering the market as finished products, which causes the price of finished products to continue to push up; the second is direct seedlings. It is very small and has no endosperm, so it is difficult to germinate and grow under natural conditions. The technical requirements for live seeding are extremely high. Although there have been successful cases, it is far from being realized for the majority of growers; therefore, white and tissue culture propagation become a top priority for development

Method used

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Examples

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Effect test

Embodiment 1

[0043] A rapid propagation and growth technology of white and tissue culture seedlings, comprising the following steps:

[0044] 1) Seed selection: select the Baiji that has been screened through the provenance as the provenance; the Baiji that has been screened through the provenance is the wild purple-flowered trigeminal Baiji in Zunyi, Guizhou;

[0045] 2) Acquisition of fruit pods: artificially cultivate fruit pods from the white seeds selected in step (1). The collection time of the fruit pods is from late October to early November, and the maturity is that the fruit pods are gray-black , to ensure that the fruit pods are not damaged; the method for obtaining the fruit pods through artificial cultivation is: digging out the pods, cleaning the soil, cutting and separating them from the main root, and smearing plant ash on the wound, and then transplanting for management and protection. Select thick flower buds, lightly dip the pollen in the flowers, and then click into the...

Embodiment 2

[0059] A rapid propagation and growth technology of white and tissue culture seedlings, comprising the following steps:

[0060] 1) Seed selection: select the Baiji that has been screened through the provenance as the provenance; the Baiji that has been screened through the provenance is the wild purple-flowered trigeminal Baiji in Zunyi, Guizhou;

[0061] 2) Acquisition of fruit pods: artificially cultivate fruit pods from the white seeds selected in step (1). The collection time of the fruit pods is from late October to early November, and the maturity is that the fruit pods are gray-black , to ensure that the fruit pods are not damaged; the method for obtaining the fruit pods through artificial cultivation is as follows: taking out the tubers of Baiji, propagating them through tubers, and obtaining the first batch of seedlings, planting the first batch of seedlings, and then planting the first batch of seedlings in the purple flower In the flowering season of Sanchabaiji, c...

Embodiment 3

[0075] A rapid propagation and growth technology of white and tissue culture seedlings, comprising the following steps:

[0076] 1) Seed selection: select the Baiji that has been screened through the provenance as the provenance; the Baiji that has been screened through the provenance is the wild purple-flowered trigeminal Baiji in Zunyi, Guizhou;

[0077] 2) Acquisition of fruit pods: artificially cultivate fruit pods from the white seeds selected in step (1). The collection time of the fruit pods is from late October to early November, and the maturity is that the fruit pods are gray-black , to ensure that the fruit pods are not damaged; the method for obtaining the fruit pods through artificial cultivation includes:

[0078] Method 1: Dig out the white root, clean the soil, cut and separate it from the main root, apply plant ash on the wound, and then transplant it for management and protection. Select thick flower buds in the flowering season, lightly dip the pollen in the...

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Abstract

The invention discloses a rapid propagation technology of rhizoma bletillae tissue culture seedlings. The rapid propagation technology includes the steps of 1), seed selection; 2), harvest of pods; 3), plant tissue culture including: A, disinfection; B, primary culture; C, subculture multiplication; D, root induction; E, acclimatization and transplant. With the technology, good provenance is guaranteed through seed selection, tissue culture time and cycle are greatly reduced through scientific tissue culture, survival rate of the tissue culture seedlings is more than 99%, and tubers obtained from culture of the tissue culture seedlings are detected to meet the standard according to Chinese pharmacopoeia.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation and growth technology of tissue culture seedlings of bletilla. Background technique [0002] Baiji is born in the mid-mountain area at an altitude of 1300-1800m. It has high ornamental value and extremely high medicinal value. Baiji is a traditional hemostatic medicine, which has the effects of stopping bleeding, reducing swelling and promoting muscle growth. Since 2000, Baiji has been indiscriminately excavated by farmers from all over the country. Coupled with the particularity of its inability to reproduce naturally, the wild Baiji is on the verge of extinction, and artificial breeding and cultivation are required. The artificial cultivation of Baiji in my country is in its infancy. , With the depletion of wild resources, the price has also risen, and the price of white and seedlings has also skyrocketed. At present, there are mainl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 张俊岳张旭
Owner 张俊岳
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