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A technique for endotracheal intubation and endotracheal intubation, which is applied in the field of endotracheal intubation of large and mice, can solve the problems of human input that is easy to be infected, easy to damage the pharynx, etc., and achieves a high success rate, avoids infection, and simple intubation method. Effect
Active Publication Date: 2018-02-27
PEKING UNIV THIRD HOSPITAL
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[0006] The purpose of the present invention is to provide a method for tracheal intubation of large and small mice, which can solve the problems of easy damage to the pharynx, easy infection and large manpower input during tracheal intubation of large and small mice
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Embodiment 1
[0053] In the present invention, the large and small mouse tracheal intubation method comprises the following steps:
[0054] Step 1. Animal preparation and equipment preparation;
[0055] Specifically, the animal preparation process is as follows: prepare the rat, place the rat in the supine position on the rat board after basic anesthesia, fix the rat's limbs and maxillary incisors with rubber bands, and raise the head of the rat board by 30°, that is, the rat's head The end where it is located is elevated by 30°;
[0056] The prepared appliances include a simple laryngoscope and a guiding set; the endotracheal tube 4 of the guiding set is connected and fixed on the operating handle 5 through a connecting tube, and the guide wire 6 is manually moved by the right hand so that the guide wire 6 extends out of the endotracheal tube 4 outside, and manually bend the end of the guide wire 6, so as not to cause damage to the tissues or organs of the rat;
[0057] Further, the spec...
Embodiment 2
[0079] In the present invention, the large and small mouse tracheal intubation method comprises the following steps:
[0080] Step 1. Animal preparation and equipment preparation;
[0081] Specifically, the animal preparation process is as follows: prepare the mouse, place the mouse in a supine position on the mouse board after basic anesthesia, fix the mouse limbs and maxillary incisors with rubber bands, and raise the head of the mouse board by 30°, that is, the head of the mouse The end where it is located is elevated by 30°;
[0082] The prepared appliances include a simple laryngoscope and a guiding set; the endotracheal tube 4 of the guiding set is connected and fixed on the operating handle 5 through a connecting tube, and the guide wire 6 is manually moved by the right hand so that the guide wire 6 extends out of the endotracheal tube 4, and manually bend the end of the guide wire 6, so as not to cause damage to the tissue or organs of the mouse;
[0083] Further, th...
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Abstract
The invention provides a tracheal intubation method for big and small mice. The method includes the following steps of preparing animals and instruments; putting a simple laryngoscope into an oral cavity of a big mouse or a small mouse to expose the glottis of the big mouse or the small mouse; inserting a tracheal catheter into the trachea of the big mouse or the small mouse under orthophoria; connecting the tail end of the tracheal catheter with a breathing machine for small animals and an anesthesia machine for the small animals, and continuing conducting inhalation anesthesia and mechanicalventilation. The tracheal intubation method for the big and small mice is wide in application range, high in success rate, applicable to molding of the big and small mice different in size, medicineapplication for tracheas, lung function detection and other occasions, and capable of avoiding the situation the the success rate of blind intubation is low, and damage is caused to the bodies of animals; the applied instruments are adaptive to the characters that the glottises of the big and small mice are narrow and the tracheas of the big and small mice are located at the deep portions of the oral cavities, pain caused by organic damage during intubation is reduced, and the stress reaction of the animals to the intubation is small; the operation is simple, and one person can complete all operation.
Description
technical field [0001] The invention relates to the field of tracheal intubation, in particular to a method for tracheal intubation of large and small mice. Background technique [0002] In medicine and biology, mice and rats are commonly used for experiments. In the experiments, it is often necessary to intubate mice and rats for mechanical ventilation or tracheal administration. Currently available intubation methods include blind intubation, tracheotomy, and direct vision intubation. [0003] The blind insertion method is easy to insert into the esophagus, the success rate is low, and it needs repeated trials. It is easy to damage the pharynx of mice and rats, resulting in the secretion of a large amount of mucus that affects the administration, and it is easy to cause infection. The pathological state increases the error of the whole experimental process. [0004] The tracheotomy method needs to incise the neck skin of rats, separate and expose the trachea layer by lay...
Claims
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