Method for synchronously detecting various coloring agents in traditional Chinese medicine preparations

A technology for simultaneous detection of traditional Chinese medicine preparations, applied in measuring devices, instruments, scientific instruments, etc., can solve the problem of no detection method, and achieve the effect of reducing detection cost, high accuracy and good stability

Inactive Publication Date: 2018-03-23
蒙小翠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among the reported liquid chromatography-mass spectrometry (LC-MS / MS) methods for the detection of synthetic dyes, they generally involve industries such as food, cosmetics, and chemicals, and there is no universally applicable detection method

Method used

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  • Method for synchronously detecting various coloring agents in traditional Chinese medicine preparations
  • Method for synchronously detecting various coloring agents in traditional Chinese medicine preparations

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Effect test

Embodiment 1

[0030] A method for simultaneous detection of multiple colorants in traditional Chinese medicine preparations, the detection method comprising the following steps:

[0031] (1) Preparation of mixed reference substance solution: Accurately weigh the appropriate amount of sunset yellow, acid red, methyl orange, golden orange II, auramine O, erythrosine and basic orange reference substances, and add methanol to prepare the mass of each component. The mixed reference solution whose fractions are all 0.1 μg / ml shall be stored in the dark at 4-6°C;

[0032] (2) Preparation of sample solution: take 0.5 g of Pueraria baicalensis slices, grind it, accurately weigh it, add 50 ml of methanol, ultrasonicate it for 40 minutes, weigh it, make up for the lost weight with methanol, and filter it; Put 1ml of the filtrate into a 10ml measuring bottle, add methanol to dilute to the mark, pass through a 0.2μm filter membrane to obtain the sample solution to be tested, and store it in the dark at ...

Embodiment 2

[0040] A method for simultaneous detection of multiple colorants in traditional Chinese medicine preparations, the detection method comprising the following steps:

[0041] (1) Preparation of mixed reference substance solution: Accurately weigh the appropriate amount of sunset yellow, acid red, methyl orange, golden orange II, auramine O, erythrosine and basic orange reference substances, and add methanol to prepare the mass of each component. The mixed reference solution whose fractions are all 0.1 μg / ml shall be stored in the dark at 4-6°C;

[0042] (2) Preparation of sample solution: take 0.5 g of Pueraria baicalensis slices, grind it, accurately weigh it, add 60 ml of methanol, ultrasonicate it for 40 minutes, weigh it, make up for the lost weight with methanol, and filter it; Put 1ml of the filtrate in a 10ml measuring bottle, add methanol to dilute to the mark, pass through a 0.2μm filter membrane to obtain the sample solution to be tested, and store it in the dark at 4-...

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Abstract

The invention discloses a method for synchronously detecting various coloring agents in traditional Chinese medicine preparations. The method is used for measuring by a liquid chromatography-mass spectrometry method. The method includes the steps: (1) mixed reference substance solution preparation; (2) sample solution preparation; (3) liquid chromatography-mass spectrometry analysis; (4) result acquisition. The various coloring agents include sunset yellow, acid red, methyl orange, gold orange II, auramine O, erythrosine and basic orange. The method has the advantages of simplicity and rapidness in operation, high accuracy and good stability and is applicable to laboratories for controlling the quality of traditional Chinese medicine preparations, batch detection can be achieved by the method, and the method is high in detection efficiency.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine detection, in particular to a method for synchronous detection of multiple colorants in traditional Chinese medicine preparations. Background technique [0002] The production of traditional Chinese medicine preparations and prepared slices of Chinese medicine is the source of the production, operation and use of Chinese patent medicines. The dyeing and adulteration of traditional Chinese medicine preparations and slices of Chinese medicine will bring serious harm to the subsequent production and clinical formulation of traditional Chinese medicine. With the popularization of traditional Chinese medicine and the development of traditional Chinese medicine preparations, the market demand for traditional Chinese medicine preparations and traditional Chinese medicine decoction pieces is increasing, and the price is rising. Driven by economic interests, the phenomenon of adulteration and fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 蒙小翠邓喜然
Owner 蒙小翠
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