Kangting lily magnolia seed tissue culture rapid propagation method

Abstract

The invention belongs to the technical field of deciduous tree planting and discloses a Kangting lily magnolia seed tissue culture rapid propagation method. Explants are collected in May; the contamination rate is 9.4% when the explants are sterilized with HgCl2 for 15 minutes; an optimal anti-browning effect is achieved when the concentration is VC 10mg / L; MS basic culture media of 6-BA 1.0mg / L and IBA 0.5mg / L are added, and thus an optimal primary culture effect is achieved; in subculture differentiation culture, the propagation coefficient of the culture medium MS+6-BA1.0mg / L+IBA1.0mg / L+NAA0.1mg / L is 4.3; rooting culture is optimally implemented with 1 / 2MS basic culture medium of IBA0.1mg / L and NAA0.1mg / L and ABT0.2mg / L, and the rooting rate is 82.93%. A transplanting medium screening test shows that under a condition that the ratio of humus soil to vermiculite is 1:1, an optimal Kangting lily magnolia tissue culture seedling growth effect is achieved, and the survival rate is up to80%.

Description

technical field [0001] The invention belongs to the technical field of planting deciduous trees, and in particular relates to a method for tissue culture and rapid propagation of Kangding magnolia seeds. Background technique [0002] Kangding Magnolia is a deciduous tree with thick, glabrous, light yellow-green twigs, papery leaves, obovate or elliptic, shiny green on the top, gray-green or white powder on the bottom, if the leaves are sometimes red, the flowers open before the leaves, large and Fragrant, tepals 9-12, obovate to narrowly ovate, 8-12cm long, white inside, pink or purple outside, seeds irregularly obovoid, black-red. It blooms first and then leaves, blooms from March to April, and bears fruit from July to September. Kangding magnolia is a unique species in Sichuan, with a very narrow distribution area, mainly distributed in the humid valleys with an altitude of 2000-2500m, such as Hailuogou, Yanzigou, Yusaping, Luding County, and Hongba, Jiulong County, in we...

AI Technical Summary

Problems solved by technology

[0003] To sum up, the problems existing in the existing technology are: the cutting and grafting technology of Kangding magnolia at present, the survival rate of the cutting technology is low, more materials are needed, and the damage to the mother tree is greater; the cutting technology is greatly affected by the season, and only It can be cut 1-2 times, but the propagation speed is slow; the seedlings obtained from cuttings have the same genetic genes, which is not conducive to the protection of biodiversity

Grafting technology is suitable for fruit trees, but not suitable for plant protection

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