Lipoprotein detection kit

Abstract

The invention relates to the technical field of medical detection, in particular to a lipoprotein detection kit. The kit comprises a R1 reagent and a R2 reagent, wherein the R1 reagent is prepared from a buffer solution, BSA (Bull Serum Albumin), chylomicron dissociation agents, surfactants, sodium chloride and preservatives; the R2 reagent is prepared from a buffer solution, lipoprotein multiresistant latex microspheres, aminopropionic acid, EDTA (Ethylene Diamine Tetraacetic Acid), BSA, sugar and preservatives. The kit can effectively eliminate the interference effect of chylomicrons in samples to be tested; the accurate determination of lipoprotein in specimens with high chylomicron contents is realized; the sensitivity is high; the linear detection range is wide.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a lipoprotein detection kit. Background technique [0002] Lipoprotein (a) (Lipoprpotein (a)) is a protein in human serum with a structure similar to LDL (low-density lipoprotein), and its density is between HDL (high-density lipoprotein) and LDL. The core part of lipoprotein (a) is neutral lipid and apoB-100 molecules, surrounded by hydrophilic apo (a), the two are covalently linked by disulfide bonds; among them, apo (a) is highly glycosylated The hydrophilic protein, the length of the peptide chain is very inconsistent, and the molecular size is obviously polymorphic. A high concentration of Lpa in serum is an indicator of arteriosclerosis and the degree of risk of heart disease. Elevated plasma lipoprotein (a) is associated with a variety of cardiovascular diseases, including peripheral vascular disease, cerebrovascular disease, premature coronary heart disease, a...

AI Technical Summary

Problems solved by technology

The reason is that lipoprotein (a) has similar characteristics to chylomicrons, and the diameter of chylomicrons is much larger than that of lipoprotein (a). For samples with high chylomicron concentration, lipoprotein (a) is easily wrapped by chylomicrons. It makes it difficult for the lipoprotein (a) in the sample to bind to the lipoprotein (a)-specific antibody in the kit components, making the measured value lower than the true value and reporting as a false negative result

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