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The Method of Improving the Yellow Seed Rate of Brassica napus by Utilizing the Aboriginal Bacteria Producing Gibberellin

A technology of Brassica napus and indigenous bacteria, applied in horticultural methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of not finding the internal reasons for the difference in seed coat color, etc., and achieve low cost and low reagent volume Less, improve the effect of yellow seed rate

Active Publication Date: 2020-10-02
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have reported the change period of rapeseed coat color and the temporal and spatial differences in seed coat color, but did not find the internal reason for the difference in seed coat color

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Obtaining indigenous gibberellin-producing bacteria: using conventional methods, the indigenous gibberellin-producing bacteria were obtained after isolation and identification from well-growing Brassica cabbage and its surrounding soil.

[0017] Expanded culture of indigenous gibberellin-producing bacteria: The above-mentioned indigenous gibberellin-producing bacteria were subjected to liquid culture. The components of the medium are: glucose 1%, corn flour 1.5%, KH2PO4 0.1%, MgSO4 0.1%, boric acid 0.02%, glycerol 0.2%, vitamin B6 0.05%, peptone 0.1%, adjust the pH value to 4.0, and sterilize the backup use. Inoculate indigenous bacteria producing gibberellin, shake slowly with a shaker, and culture at 28°C for 3 days. The above-mentioned culture solution is filtered with sterile gauze, and diluted 7 times after filtration according to the concentration of the bacteria, and the pH value is adjusted to 5.5-6.5 for later use, referred to as the reserve solution.

Embodiment 2

[0019] Get potted cabbage-type yellow-seed rape plants under natural conditions. When the plants bloom for the first time for 3 days, get the standby solution in Example 1 and spray rape flowers and siliques. The local weather conditions are no rain within 48 hours. After the seeds are ripe and harvested, the test shows that the content of anthocyanidin and proanthocyanidin in the seed coat has decreased by 30% and 53% respectively, and the yellow seed rate has increased by 42%.

Embodiment 3

[0021] Get potted cabbage-type yellow-seed rape plants under natural conditions. When the plants bloom for the first time for 5 days, get the standby solution in Example 1 and spray rape flowers and siliques. The local weather conditions are no rain within 48 hours. After the seeds are ripe and harvested, the test shows that the content of anthocyanidin and proanthocyanidin in the seed coat has decreased by 41% and 60% respectively, and the yellow seed rate has increased by 45%.

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PUM

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Abstract

The invention relates to a method for increasing cabbage type rape yellow seed rate by indigenous bacteria generating gibberellin. The method includes the steps: viewing local weather forecast to ensure that rain is omitted within 48 hours after the method is implemented; spraying flowers and siliques of the cabbage type yellow seed rapes of 3-15 days after first blossoming by the aid of mixed liquid of the indigenous bacteria generating the gibberellin; harvesting the rapes when seeds are mature. After the seeds are mature and harvested, the anthocyanidin content and the proanthocyanidin content of seed coats of the rapes are reduced by 25-45% and 50-60%, and the yellow seed rate can be increased by 30-45%.

Description

technical field [0001] The invention relates to the field of agricultural applied biotechnology, in particular to a method for improving the yellow seed rate of Brassica napus by using indigenous bacteria producing gibberellin. Background technique [0002] Studies have shown that under the same genetic background, compared with black seed rape, yellow seed rape has the advantages of thin seed coat, high oil content, high protein content, less pigment in oil and transparency. Because the properties of yellow seeds in Brassica napus are unstable, the rate of yellow seeds decreases, and some yellow seeds will reverse to black seeds, etc., so certain cultivation and management measures are taken to obtain rapeseed products with less pigment in the seed coat and high rate of yellow seeds It has always been one of the concerns in rapeseed cultivation. [0003] From the perspective of the development and composition of rapeseed, the embryos of both yellow-seed rapeseed and black-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/15A01G7/06C12N1/00A01N63/20A01N63/30A01P21/00
CPCA01G7/06A01N63/00C12N1/00
Inventor 严明理高国应伍小芳张大为刘丽莉
Owner HUNAN UNIV OF SCI & TECH