Method for breeding polygene polymeric two-line sterile line suitable for use by mechanized mixed-planting seed production
A multi-gene, suitable technology, applied in the field of agriculture, can solve the problems that restrict the application and promotion of hybrid rice mechanized seed production technology, cannot guarantee the quality of hybrid seeds, and the color difference of chaff is not obvious, so as to improve the efficiency of seed production and improve the quality of breeding. Efficiency, the effect of reducing the cost of color sorting
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[0025] In the following examples, there is a brown marker 'Xin'an S' and a dominant semi-dwarf gene SD-d(t) And the excellent insensitivity to gibberellin 'Quan 151S' is an excellent two-line male sterile line bred by Anhui Quanyin High-Tech Seed Industry Co., Ltd.; the herbicide-resistant material is the conventional material 'Jietian Rice' provided by Xingwang Biotechnology Co., Ltd. 001'; herbicides mainly refer to imidazolinone herbicides, and the herbicide used in this example is imazethapyr produced by BASF Europe, commonly known as "Bailongtong".
[0026] The invention provides a method for breeding sterile lines suitable for multigene aggregation used in mechanized mixed sowing seed production, comprising the following steps:
[0027] (1) Based on the hybrid rice parent 'Xin'an S' with a recessive brown marker, and a herbicide resistance gene ALS The rice 'Jietiandao 001' was crossed to obtain the hybrid F 1 ;
[0028] (2) Using a combination of conventional breed...
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