A two-line sterile line breeding method suitable for multi-gene aggregation used in mechanized mixed seed production

Abstract

The invention discloses a method for breeding a polygene polymeric two-line sterile line suitable for use by mechanized mixed-planting seed production. After rice parents with a recessive brown or pocked or black-brown specific marker are hybridized with a good two-line sterile line of rice which contains a herbicide-resistant endogenous gene and a dominant semi-dwarf gene and is insensitive to gibberellin, selfing is carried out, a restorer is utilized for combination testing, and a two-line sterile line with high combining ability, the genes and performance characteristics is chosen out. Themethod realizes polygene polymerization of the two-line sterile line; in the process of carrying out mixed-planting seed production with a combination of the two-line sterile line, after pollinationis complete, a corresponding herbicide is sprayed to kill a male parent, guaranteeing the production of qualified hybrid seeds; moreover, since the two-line sterile line is insensitive to the gibberellin, the defects of overhigh plants and difficult pollination caused by the spraying of gibberellin in the process of seed production are overcome, and therefore the yield of seed production is increased.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a two-line male sterile line breeding method suitable for multigene aggregation used in mechanized mixed sowing seed production. Background technique [0002] With the development of photoelectric color sorting technology, by breeding specific female parents (sterile lines), mixed harvesting of male parents and female parents can be realized, and sorting after mixed harvesting can be realized. At present, the color sorting machinery and equipment are sorted by the color difference of the chaff. To achieve high-precision sorting, the chaff color difference of the parents’ seeds needs to be large. The smaller the proportion of male seeds, the higher the accuracy of color sorting and the better the purity. Patent CN 1454463A discloses a breeding method for hybrid rice parents and varieties with recessive markers, which provides basic materials and technical support for breedi...

AI Technical Summary

Problems solved by technology

[0003] At present, gibberellin is sprayed for mixed seed production of hybrid rice, which causes the plant height of the female parent to be too high or even higher than that of the male parent, making pollination difficult

[0004] The color difference between the parents of most hybrid rice varieties is not obvious, and the quality of hybrid seeds cannot be guaranteed through photoelectric color sorting, which restricts the application and promotion of hybrid rice mechanized seed production technology

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