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Actinomycetes and application thereof

A technology for actinomycetes and inoculants, applied in the field of microorganisms, can solve problems such as damage to the soil ecological environment, human insecurity, and outstanding drug resistance of pathogenic bacteria, and achieve the effect of significant antagonistic effect, good development and application prospects, and broad development space.

Active Publication Date: 2018-06-01
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some cultivation management measures can only play a partial control role
And the long-term use of some chemical fungicides and modifiers can easily lead to the problem of drug resistance of pathogenic bacteria, and damage the ecological environment of the soil, which is not safe for humans

Method used

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  • Actinomycetes and application thereof
  • Actinomycetes and application thereof
  • Actinomycetes and application thereof

Examples

Experimental program
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Embodiment Construction

[0013] The present invention will be further described below in conjunction with specific examples, so as to better understand the present invention.

[0014] The invention provides an actinomycete, which is Streptomyces manipurensis-1 (streptomyces manipurensiss-1), the preservation number is CCTCC No: M2017261, the preservation date is May 15, 2017, and the preservation unit is China Typical Culture Object Preservation Center (Address: Wuhan University, Wuhan, China). Streptomyces manipurensis s-1 (streptomyces manipurensis s-1) of the present invention is isolated from well-growing banana orchard soil.

[0015] 1 strain identification

[0016] 1.1 16S rDNA sequence analysis

[0017] Bacterial 16S rDNA universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3') were used to establish a PCR amplification system for amplification (see Table 1 for PCR reaction conditions). After the product was purified, the gene sequence was determined; the sequ...

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Abstract

The invention provides actinomycetes which is streptomyces manipurensis S-1 and is assigned the accession number CCTCC No: M2017261. The streptomyces manipurensis S-1 is 5.0-10.0 in growth pH range, preferably 7.0, and has optimum growth temperature of 28-32 DEG C and cannot grow on a culture medium being more than 3% in content of NaCl. The streptomyces manipurensis S-1 has an antagonism effect against Fusarium oxysporum f. sp. cubense (FOC) race 1 and 4, and especially has significant antagonism effect against Fusarium oxysporum f. sp. cubense (FOC) race 4. The streptomyces manipurensis S-1has extensive development space and has great development and application prospects.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an actinomycete and its application. Background technique [0002] Banana Fusarium wilt, also known as Panama disease and banana yellow leaf disease, is a vascular wilting disease caused by Fusarium oxysporum f.sp.cubense. The pathogen invades from the root wound of the host, and spreads to the upper leaves through the corm and pseudostem through the vascular bundle. The pathogen blocks the xylem vessel, hinders the water transportation of the plant, and causes the plant to wither and die. This devastating soil-borne disease, once a field develops, the germs spread quickly and are difficult to cure, so it is called "banana cancer". Therefore, it is imminent to control banana wilt. [0003] At present, there is no effective control method for the disease, and there are no ideal chemical agents and high-quality disease-resistant varieties. Some cultivation management me...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/04A01P3/00C12R1/465
CPCA01N63/30C12N1/20C12N1/205C12R2001/465
Inventor 段雅婕庞振才陈晶晶胡会刚李伟明胡玉林孙德权谢江辉
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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