Vitis amurensis SNP molecular markers and application thereof in genetic map construction and white rot resistance location

Abstract

Belonging to the technical field of plant molecular marker preparation and application, the invention specifically relates to vitis amurensis SNP molecular markers and application thereof in genetic map construction and white rot resistance location. The sum of the molecular markers is 7124, and the positional information of the molecular markers on grape chromosomes is distributed to 19 chromosomes. According to the invention, by establishing an SNP marking technology developed based on SLAF-seq (specific length amplified fragment-sequencing) technology, a large number of SNP markers with gene sequences are obtained, a vitis amurensis high density molecular genetic map is constructed, and location study is carried out grape white rot resistance so as to obtain grape white rot resistance related SNP markers, and the functional markers have good application prospects in grape white rot resistant molecular marker assisted breeding.

Description

technical field [0001] The invention belongs to the technical field of preparation and application of plant molecular markers, in particular to a vine SNP molecular marker and its application in genetic map construction and white rot resistance positioning. Background technique [0002] Grape (Vitis vinifera) is one of the world's important fruit trees with high nutritional value. In recent years, people's consumption of high-quality grapes has been increasing. In 2013, the world's total output was 67.067 million tons, and the cultivated area was about 7 million hectares. On the other hand, with the intensification of pests and diseases and various natural disasters, the growth and development of grapes and the improvement of quality and yield are seriously affected. Therefore, improving the important quality traits and stress resistance traits of grapes has always been an important goal of grape breeders. . [0003] Researchers select germplasm resources with excellent cha...

AI Technical Summary

Problems solved by technology

In the northern regions of my country, the annual loss rate of grape fruit caused by white rot is about 20%. If the white rot is not well controlled, the disease will seriously damage the fruit and ears during the period of prevalence, causing inestimable losses. even lost

In view of the fact that the construction of a high-density molecular genetic map of grapes has not been seen in the prior art, it is particularly important to construct a high-density molecular genetic map of grape white rot resistance genes for the localization research of grape white rot resistance genes

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