A kind of nano liposome carrying polypeptide and its preparation method and application
A nanoliposome and targeting peptide technology, applied in the field of biomedicine, can solve the problems of unsatisfactory clinical treatment effect, insoluble curcumin in water, low cell uptake rate, etc., and achieve inhibition of experimental spontaneous encephalomyelitis. production, excellent physical and chemical properties, and good biocompatibility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] Embodiment 1 Preparation of Nanoliposomes Carrying Polypeptide
[0041] The main components of the nanoliposome in this example are targeting polypeptide R4F, phospholipids, and cholesterol oleate, wherein the sequence of the targeting peptide R4F is FAEKFKEAVKDYFA KFWDGSG (SEQ ID No.1, derived from C57 strain small mouse myelin antigen polypeptide), the steps for preparing nanoliposomes carrying the polypeptide are:
[0042] 1) Fully mix 2mg of dimyristoylphosphatidylcholine (DMPC) and 0.065mg of cholesterol oleate in a glass test tube, and seal the test tube with a parafilm;
[0043] 2) dry up the chloroform in the test tube in a steady stream of nitrogen, so that the mixture in step 1) can form a thin film at the bottom of the test tube;
[0044] 3) Put the test tube into a vacuum desiccator and dry it under vacuum for 1 hour;
[0045] 4) Add 2ml of phosphate buffer solution (pH 7.2) to the test tube, fill it with nitrogen gas and seal it, and use a vortex shaker t...
Embodiment 2
[0049] Embodiment 2 carries the preparation of the nano liposome of curcumin
[0050] In this embodiment, the main constituents of the nanoliposomes carrying curcumin are targeting polypeptide R4F, phospholipids, curcumin, and cholesterol oleate, wherein the sequence of the targeting peptide R4F is as shown in SEQ ID No.1 Show, the step of preparing the nano-carrier that carries curcumin is:
[0051] 1) Mix 2mg of dimyristoylphosphatidylcholine (DMPC), 0.065mg of cholesterol oleate (CO), and 0.3mg of curcumin in chloroform; mix well in a glass test tube, and seal the test tube with a parafilm;
[0052] 2) dry up the chloroform in the test tube in a steady stream of nitrogen, so that the mixture in step 1) can form a thin film at the bottom of the test tube;
[0053] 3) Put the test tube into a vacuum desiccator and dry it under vacuum for 1 hour;
[0054] 4) Add 2ml of phosphate buffer solution (pH 7.2) to the test tube, fill it with nitrogen gas and seal it, and use a vorte...
Embodiment 3
[0059] Example 3 Preparation of Nanoliposomes Carrying DIRBOA
[0060] In this embodiment, the nanoliposomes carrying the fluorescent dye DIRBOA (lipophilic carbocyanine dye) mainly consist of targeting polypeptide R4F, phospholipids, DIRBOA, and cholesterol oleate, wherein the targeting peptide R4F The sequence is as shown in SEQ ID No.1, and the steps for preparing a nanocarrier carrying DIRBOA are:
[0061] 1) Dissolve 2mg of dimyristoylphosphatidylcholine (DMPC), 0.065mg of cholesterol oleate (CO), and 0.2mg of DIRBOA in chloroform solution; mix well in a glass test tube, and seal the test tube with a parafilm;
[0062] 2) dry up the chloroform in the test tube in a steady stream of nitrogen, so that the mixture in step 1) can form a thin film at the bottom of the test tube;
[0063] 3) Put the test tube into a vacuum desiccator and dry it under vacuum for 1 hour;
[0064] 4) Add 2ml of phosphate buffer solution (pH 7.2) to the test tube, fill it with nitrogen gas and se...
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle size | aaaaa | aaaaa |
| particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More