Culture method of silkworm chrysalis cordyceps sinensis

A technology of Cordyceps sinensis and a cultivation method, applied in the field of Cordyceps militaris cultivation, can solve the problems of low survival rate, great difficulty in planting and the like

Inactive Publication Date: 2018-06-29
泰安皇草生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, silkworm chrysalis Cordyceps sinensis is produced by biotechnology artificial planting with insect pupa bacteria, which is difficult to plant and has a low survival rate, and the content of nutritional and functional components of silkworm chrysalis Cordyceps sinensis needs to be further improved

Method used

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  • Culture method of silkworm chrysalis cordyceps sinensis
  • Culture method of silkworm chrysalis cordyceps sinensis

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A method for cultivating silkworm chrysalis Cordyceps sinensis, comprising the following steps:

[0024] 1) Culture medium preparation: select the high-quality green silkworm chrysalis from the pollution-free area of ​​the Rizhao seashore, soak it in the culture solution for 40 minutes, and then pack it into a glass culture bottle to obtain the culture medium;

[0025] The formula ratio of the culture solution is: glucose 12g, peptone 5g, potassium dihydrogen phosphate 3g, magnesium sulfate 1g, add water to 1000mL;

[0026] 2) Sterilization: Seal the culture bottle and put it into an autoclave for sterilization, heat up to 100°C, close the exhaust valve, keep it for 1 hour when the pressure of the autoclave reaches 1.5 bar, take it out, and cool it down;

[0027] 3) Inoculation: under aseptic conditions, spray the liquid strain on the surface of the culture medium with an inoculation gun. The density of the liquid strain is 5 bacterial balls / mL, and the mass ratio of th...

Embodiment 2

[0034] A method for cultivating silkworm chrysalis Cordyceps sinensis, comprising the following steps:

[0035] 1) Culture medium preparation: select the high-quality green silkworm chrysalis from the pollution-free area of ​​the Rizhao seashore, soak it in the culture solution for 30 minutes, and then pack it into a glass culture bottle to obtain the culture medium;

[0036] The formula ratio of the culture solution is: glucose 15g, peptone 4g, potassium dihydrogen phosphate 4g, magnesium sulfate 0.5g, add water to 1000mL;

[0037] 2) Sterilization: Seal the culture bottle and put it into an autoclave for sterilization, heat up to 100°C, close the exhaust valve, keep it for 1 hour when the pressure of the autoclave reaches 1.5 bar, take it out, and cool it down;

[0038] 3) Inoculation: under aseptic conditions, spray the liquid strain on the surface of the culture medium with an inoculation gun. The density of the liquid strain is 3 bacterial balls / mL, and the mass ratio of ...

Embodiment 3

[0045] A method for cultivating silkworm chrysalis Cordyceps sinensis, comprising the following steps:

[0046] 1) Culture medium preparation: select the high-quality green silkworm chrysalis from the pollution-free area of ​​the Rizhao seashore, soak it in the culture solution for 40 minutes, and then pack it into a glass culture bottle to obtain the culture medium;

[0047]The formula ratio of the culture solution is: glucose 10g, peptone 7g, potassium dihydrogen phosphate 2g, magnesium sulfate 2g, add water to make it to 1000mL;

[0048] 2) Sterilization: Seal the culture bottle and put it into an autoclave for sterilization, heat up to 100°C, close the exhaust valve, keep it for 1 hour when the pressure of the autoclave reaches 1.5 bar, take it out, and cool it down;

[0049] 3) Inoculation: under aseptic conditions, spray the liquid strain evenly on the surface of the culture medium with an inoculation gun. The density of the liquid strain is 4 bacterial balls / mL, and the...

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Abstract

The invention relates to the technical field of a culture method of cordyceps militaris. The culture method comprises the following steps of 1, preparation of a culture medium; 2, sterilization; 3, inoculation; 4, management, wherein management comprises growth of mycelia, sprouting of sporocarp and growth of the sporocarp; 5, harvesting. Silkworm chrysalis cordyceps sinensis is plump in body andgolden in color, as is identified, main nutrient components of silkworm chrysalis cordyceps sinensis are higher than those of wild cordyceps sinensis, the harvested cordyceps sinensis sporocarp is immediately subjected to low temperature quick-freezing sterilization and drying, so that all fresh nutrient components are reserved and cannot get lost, in the culture process, plant enzymes of different attributes are injected, five-element cordyceps militaris sporocarp with different emphasized efficacies are obtained, thus all natural alkaline healthcare food is made, the efficacies of health maintenance, healthcare, prevention and treatment are achieved, and cordyceps militaris is an ideal raw material for making relevant medicine and healthcare products.

Description

technical field [0001] The invention relates to the technical field of cultivation methods of Cordyceps militaris, in particular to a cultivation method of silkworm chrysalis Cordyceps sinensis. Background technique [0002] Wild Cordyceps sinensis, which is scarce in resources, has been in short supply in the market as a traditional precious traditional Chinese medicine and tonic. Cordyceps sinensis, a silkworm chrysalis cultivated from silkworm chrysalis, is a medicinal fungus combined with silkworms and wild Tibetan fungi. Silkworm chrysalis Cordyceps sinensis is not only rich in protein and amino acids, cordycepin, cordycepic acid, and polysaccharides, but also contains more than 30 trace elements needed by the human body. It contains a variety of physiologically active ingredients that have anti-tumor, anti-bacterial, anti-oxidation, and liver protection , enhance immunity and other pharmacological effects. Domestic and foreign studies on the species that have induced...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20C12N1/14A23L31/00C12R1/645
CPCC12N1/14
Inventor 庄輝宏陈春莲
Owner 泰安皇草生物医药科技有限公司
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