Red root wild broad bean stem section tissue culture seedling culture method

Abstract

The invention discloses a red root wild broad bean stem section tissue culture seedling culture method, which comprises the following steps including an explant selection step: seeding wild broad beanseeds; then putting the seeds into a culture bottle; performing subculture until the seedlings grow to 10 to 12 cm; obtaining primary bottle seedlings; an inoculation step: taking out the primary bottle seedlings; performing sterilization; taking out seedlings; cutting leaves and leaf stems on the seedling stems; then, cutting the seedling stems into a plurality of stem sections, wherein each stem section at least comprises one axillary bud; then, transplanting a plurality of stem sections onto 1 / 2 MS culture medium to be cultured to obtain inoculation stem sections; a multiplication culturestep: transferring the inoculation stem sections into a culture chamber to be cultured so that stem section lateral buds grow to 3 to 5 cm; obtaining secondary bottle seedlings; a rooting culture step: transferring the secondary bottle seedlings into a rooting culture medium to be cultured to obtain the rooting bottle seedlings. The red root wild broad bean stems are used and are cut into small sections for multiplication; the quantity of the red root wild broad bean seedling can be fast increased; the current condition of rare resources can be relieved.

Description

Technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for culturing seedlings of red-rooted wild broad bean stems. Background technique [0002] Red-rooted broad bean, angiosperm, dicotyledon, Scrophulariaceae, and flax genus in the plant kingdom, is the whole plant centranthera cochinchinensis (lour.)merr.[digitaliscochinchinensis lour.], and Names: flax sesame, Huaxue Dan, Xiaohongyao, Fava Bean, etc.; warm in nature, sour in taste, slightly pungent, used to relieve blood stasis, stop bleeding, reduce swelling and pain; Indications for hemoptysis, hemoptysis, vomiting, women's amenorrhea, internal injuries Bleeding, middle-aged and elderly rheumatic arthralgia, muscle and bone pain, cerebral congestion, cerebral thrombosis, alcoholic liver, etc. Red-rooted wild broad bean is a kind of precious local ethnic Chinese medicine, mainly distributed in Vietnam, Yunnan, Honghe and Wenshan prefectures on the border of Vietnam. ...

AI Technical Summary

Problems solved by technology

Red-rooted wild broad bean is a precious local traditional Chinese medicine. It is mainly distributed in Vietnam, Honghe Prefecture and Wenshan Prefecture in Yunnan near the Vietnamese border. At present, there are few wild resources of red-rooted wild broad bean. People excavate it too much, and there are seeds that reproduce naturally. Low capacity, difficult to survive after transplanting, etc., lead to scarcity of wild resources of red root wild broad bean

[0003] The whole plant of red root wild broad bean has medicinal value, which is recorded in "Yunnan Simao Chinese Herbal Medicine Selection" and "Xinhua Materia Medica, Yunnan Honghe Chinese Herbal Medicine Handbook"; due to the small size of the seeds, the seeds have low reproductive ability and slow reproduction speed through traditional methods , under natural conditions, the emergence rate of direct seeding is about 20%-30%, and it can only grow to a height of 2-3 cm in the first year. The growth cycle is 3-5 years, and the growth cycle is long, which cannot meet the needs of medication.

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