Culture medium and application thereof

A medium and proliferation culture technology, applied in the field of microorganisms, can solve the problem of non-uniform bacterial system environment, and achieve the effect of reducing damage or inhibiting growth, simple formula and good growth state

Inactive Publication Date: 2018-06-29
康盛时代(北京)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Another technical problem to be solved by the present invention is to overcome the inconsistency of the environment of the bacterial system due to the large difference in the composition of the culture medium in the production process of using the mixed strain of fungi and bacteria in the prior art, and the composition of the culture medium inhibits other bacteria. problem of multiplication and production, and then provide a medium that can be used for the growth, reproduction and production of fungi and bacteria at the same time, so that when fungi and bacteria are used in combination, they can benefit from the unity of various species in a growth environment.

Method used

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  • Culture medium and application thereof
  • Culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A fungus and bacteria culture medium, which is composed of the following raw materials in the weight ratio: peptone 7g, glucose 25g, sodium chloride 1g, V B 0.1g, 1000g distilled water.

[0038] The configuration method is as follows:

[0039] a. Take 300g of distilled water and heat it to boiling;

[0040] b. Separate peptone, glucose, sodium chloride and V B Dissolved in boiling water;

[0041] c. Then add distilled water to 1000g, and adjust the pH to 6.

[0042] d. After sterilizing at 121°C for 20 minutes, a liquid culture medium for fungi and bacteria is obtained.

Embodiment 2

[0044] A fungal and bacterial culture medium, which is composed of the following raw materials in the weight ratio: peptone 7.5g, glucose 24g, sodium chloride 2g, V B 0.1g, 1000g distilled water.

[0045] The configuration method is as follows:

[0046] a. Take 300g of distilled water and heat it to boiling;

[0047] b. Separate peptone, glucose, sodium chloride and V B Dissolved in boiling water;

[0048] c. Then add distilled water to 1000g, and adjust the pH to 6.

[0049] d. After sterilizing at 121°C for 15 minutes, a liquid culture medium for fungi and bacteria is obtained.

Embodiment 3

[0051] A fungal and bacterial culture medium, which is composed of the following raw materials in the weight ratio: peptone 8g, glucose 20g, distilled water 1000g, sodium chloride 0.2g V B 0.05g, 10g agar.

[0052] The configuration method is as follows:

[0053] a. Take 300g of distilled water and heat it to boiling;

[0054] b. Dissolve the weighed agar in boiling water and heat until the solution is transparent;

[0055] c. Separate peptone, glucose, sodium chloride and V B Add and dissolve;

[0056] d. Add distilled water to 1000g and adjust the pH to 7.

[0057] e. After sterilizing at 121°C for 15 minutes, a solid medium for fungi and bacteria is obtained.

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Abstract

The invention discloses a fungal and bacterial culture medium. The culture medium comprises the following raw materials in parts by mass: 7-8 parts of peptone, 20-25 parts of glucose, 1-3 parts of sodium chloride, 0.05-0.2g parts of vitamin B and 1000 parts of water. The culture medium provided by the invention overcomes the defect that the conventional culture medium can only culture a single culture, namely bacteria or fungi, and is the culture medium widely applicable to the bacteria and the fungi; when the culture medium is used for culturing the bacteria or the fungi, the fungi and the bacteria grow well, even grow better that those in a specific culture medium; in addition, the culture medium is simple and easily available in formula and low in cost, and is suitable for needs of large-scale industrial production.

Description

Technical field [0001] The invention relates to the technical field of microorganisms, in particular to a culture medium and its application. Background technique [0002] The culture medium is an artificially formulated nutrient for the growth and maintenance of microorganisms, plant tissues and animal tissues. For microorganisms, different microorganisms have different requirements for nutrients, and the medium required to cultivate them is different. For example, Sabouraud medium and PDA medium are commonly used fungal culture media, but they are not conducive to the growth of bacteria; beef extract peptone medium (or ordinary broth medium for short) is a commonly used bacterial culture medium. [0003] The microorganisms can only grow well when the nutrient concentration in the medium is appropriate. When the nutrient concentration is too low, it cannot meet the needs of the normal growth of microorganisms. When the concentration is too high, it may inhibit the growth of micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/16C12N1/20C12R1/645C12R1/01C12R1/39C12R1/125C12R1/08
CPCC12N1/14C12N1/16C12N1/20
Inventor 王福喜
Owner 康盛时代(北京)生物技术有限公司
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