Method for screening extracellular electricity-producing bacteria with high electricity-producing capacity in anaerobic activated sludge
A technology of activated sludge and electrogenic bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of inability to accurately screen out electrogenic bacteria, achieve efficient and accurate screening, and the method is simple and easy line effect
Inactive Publication Date: 2018-07-06
XUZHOU NORMAL UNIVERSITY
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Patent CN201210059296 discloses a method for screening electrogenic microorganisms, but the microorganisms screened by this method are not all electrogenic bacteria, nor can it accurately screen out electrogenic bacteria with high electricity production capacity for subsequent induction and domestication work
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Embodiment 1
[0032] Such as figure 1 As shown, a method for screening extracellular electrogenic bacteria with high electricity production capacity in anaerobic activated sludge,
[0033] S1: Take the anaerobic activated sludge from the domestic sewage treatment plant into the sterilized PE pipe, and store it in a refrigerator at 4°C until use. Prepare 200ml of conventional LB liquid medium, and pass nitrogen gas for 15 minutes to remove oxygen in the medium. Take 0.2g of activated sludge in the anaerobic workstation and add it to the LB liquid medium, place it on the shaker in the anaerobic workstation for anaerobic cultivation. The rotation speed of the shaker was 190 rpm, the culture temperature was 30° C., and the culture time was 48 hours. After the cultivation, the LB liquid medium changed from clarification to turbidity.
[0034] S2: Gradiently dilute the bacterial solution in the anaerobic workstation, and the dilution is 10 -4 、10 -5 、10 -6 Gradient, the bacterial solution o...
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The invention provides a method for screening extracellular electricity-producing bacteria with high electricity-producing capacity in anaerobic activated sludge. According to the principle that the extracellular electricity-producing bacteria can reduce Fe<3+> to Fe in ferric citrate, anaerobic bacteria in the anaerobic activated sludge are separated and purified by a ferric citrate solid medium,according to the size of the blackening range of the medium surrounding a bacterial colony, some extracellular electricity-producing bacteria with high electricity-producing capacity can be initiallyselected, the initially-selected extracellular electricity-producing bacteria with high electricity-producing capacity can be determined and compared by a cyclic voltammetry method, finally optimal extracellular electricity-producing bacteria with high electricity-producing capacity can be screened to lay a foundation for subsequent induction and domestication of the electricity-producing bacteria. The method is simple and easy, and can efficiently and accurately screen out the extracellular electric-producing bacteria with high electricity-producing capacity in the anaerobic activated sludge.
Description
technical field [0001] The invention relates to the field of screening extracellular electrogenic bacteria, in particular to a method for screening extracellular electrogenic bacteria with high electricity production capacity in anaerobic activated sludge. Background technique [0002] Anaerobic activated sludge is a general term for anaerobic microbial groups and the organic and inorganic substances they are attached to. Anaerobic activated sludge contains abundant anaerobic microorganisms, suitable for screening extracellular electrogenic bacteria. Extracellular electrogenic bacteria have the ability to directly transfer electrons to compounds or indirect electron acceptors, and most of them are anaerobic or facultative anaerobic bacteria. Many anaerobic bacteria can only transfer electrons to soluble exogenous compounds, such as nitrate or sulfate, which can enter the interior of the cell membrane by diffusion. Extracellular electrogenic bacteria have the ability to tran...
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IPC IPC(8): C12N1/02C12N1/20C12Q1/04C12R1/01
CPCC12N1/02C12N1/20C12Q1/04
Inventor温洪宇王国振王梦影
OwnerXUZHOU NORMAL UNIVERSITY