Screening method for degrading feather strain and its application method in degrading feather

An application method and strain technology, applied in the field of microorganisms, can solve the problems of restricting the application and popularization of the technology, destroying the nutritional value of feather meal, and low utilization rate of keratin, achieving broad prospects for industrial production, improving absorbability and cultivating raw materials. Simple and accessible effects

Active Publication Date: 2021-02-09
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have great disadvantages in the process of processing, such as: high investment cost, high energy consumption, environmental pollution, and the nutritional value of the feather meal obtained is destroyed in varying degrees, resulting in low keratin utilization.
These seriously restrict the application and popularization of technology

Method used

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  • Screening method for degrading feather strain and its application method in degrading feather
  • Screening method for degrading feather strain and its application method in degrading feather
  • Screening method for degrading feather strain and its application method in degrading feather

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the screening 1 of feather degrading strain

[0035] The discarded feathers and the soil of feather accumulation sites were collected from a slaughterhouse in Lushun.

[0036] ABTNL-1 strain was obtained through enrichment culture, primary screening, secondary screening, and further isolation and purification.

[0037] Test rig and feather handling

[0038] Experimental device for crushing feathers: 220V, 3.6KW high-speed multifunctional grinder.

[0039] Feather treatment: The feathers collected at the live poultry slaughter point are rinsed with running water, dried at 55°C for 5-7 days, and set aside. Feathers are pulverized by a pulverizer and passed through a 100-mesh sieve for solid culture.

[0040] Preparation of enrichment medium: Weigh 5.0g of beef extract, 10.0g of peptone, 5.0g of sodium chloride, and 1.0g of dipotassium hydrogen phosphate, add them to a conical flask containing 1000ml of distilled water, stir and dissolve on a magnetic stir...

Embodiment 2

[0051] Embodiment 2: the screening 2 of feather degrading strain

[0052] The discarded feathers and the soil of feather accumulation sites were collected from a slaughterhouse in Lushun.

[0053] ABTNL-1 strain was obtained through enrichment culture, primary screening, secondary screening, and further isolation and purification.

[0054] Test rig and feather handling

[0055] Experimental device for crushing feathers: 220V, 3.6KW high-speed multifunctional grinder.

[0056] Feather treatment: The feathers collected at the live poultry slaughter point are rinsed with running water, dried at 55°C for 5-7 days, and set aside. Feathers are pulverized by a pulverizer and passed through a 100-mesh sieve for solid culture.

[0057] Preparation of enrichment medium: Weigh 5.0g of beef extract, 10.0g of peptone, 5.0g of sodium chloride, and 1.0g of dipotassium hydrogen phosphate, add them into a conical flask containing 1000ml of distilled water, stir and dissolve on a magnetic st...

Embodiment 3

[0068] Embodiment 3: strain identification

[0069] The above-mentioned high-efficiency degradation feather strain identification process of the present invention is specifically as follows:

[0070] Identification of strains: use 16SrRNA method to identify microbial strains

[0071] Bacteria identification results: PCR amplification and sequencing: the strain was cultured to the exponential growth phase with broth medium as the substrate, the bacteria were collected by centrifugation, and the total DNA was obtained by boiling with a PCR machine. The primers used in the 16S rRNA PCR reaction system were universal Primers (27F: 5-AGAGTTTGATCCTGGCTCAG-3, 1492R: 5-GGTTACCTTGTTACGACTT-3,).

[0072] PCR reaction system

[0073]

[0074] PCR reaction program: pre-denaturation at 94°C for 5 min; denaturation at 94°C for 1 min, annealing at 56°C for 1 min, extension at 72°C for 2 min; cycle 29 in the second step; 10 min at 72°C, electrophoresis observation. The PCR products were...

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Abstract

The invention relates to a method for degrading feather waste by utilizing microbial strains, and belongs to the technical field of microorganisms. The specific steps of the method are as follows: screening of bacterial strains; treatment of feathers: wash the feathers with clean water and put them into an oven at 45°C-55°C, and dry them for 4-5 days for later use; After high temperature and high pressure sterilization, inoculate the microbial strain ABTNL-1, and cultivate it under the condition of 37°C and 160r / min, and the degradation rate can reach 85% in 36 hours. The use of amino acids and polypeptides reduces environmental pollution. In addition, microbial degradation of feathers, compared with physical and chemical methods, does not require equipment, which greatly reduces energy consumption. At the same time, its degradation efficiency is high, the fermentation cycle is short, and the loss of most amino acids is avoided, which improves the absorbability of feather degradation products. availability.

Description

technical field [0001] The invention belongs to the technical field of microbes, and more specifically relates to the screening of highly efficient feather-degrading bacterial strains and a method for degrading discarded feathers. Background technique [0002] The data shows that globally, the annual output of feathers is about 8.5 billion tons. In China, the annual output of feathers can be as high as 11 million tons; while the annual output of discarded feathers can be as high as 100,000 tons; feathers belong to natural keratin, and the horns in feathers The protein content is as high as about 90%, and keratin is a hard protein, which is difficult to be digested by the digestive enzymes in the gastrointestinal tract, so it has not been efficiently utilized for a long time, resulting in a waste of available resources. [0003] In recent years, with the rapid development of scale and intensification of the breeding industry, more than 10,000 tons of feather waste such as fea...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/04C12N1/02C12P21/00C12R1/10
CPCC12N1/02C12N1/04C12P21/00
Inventor李晓宇贾藏藏徐永平王丽丽陈岩周通曲芳京
OwnerDALIAN UNIV OF TECH