White tea manufacturing time determination method based on year white tea characteristic compound
A technology of characteristic compounds and identification methods, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of human subjectivity, tea identification, and obstacles to the development of white tea tea industry, etc., to achieve fast experimental operation and data analysis, and reduce requirements , the objective effect of the result
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[0026] Identification of the age of white tea: through the identification of 8- C N -Ethyl-2-pyrrolidone substituted epigallocatechin gallate content was determined, and then a linear discriminant model was established with the year, so as to identify the age of the unknown white tea samples.
[0027] Specific steps:
[0028] 1) Collect 18 samples of Baihao Yinzhen white tea and 26 samples of Baimudan white tea from different years, grind them into powder and store them at -20°C.
[0029] 2) Determination of 8- C N - Content of ethyl-2-pyrrolidone substituted epigallocatechin gallate:
[0030] Accurately weigh 0.1 g of white tea sample powder into a centrifuge tube, add 70% methanol aqueous solution and extract in a 70°C water bath for 30 minutes, then centrifuge in a high-speed centrifuge at 10,000 rpm for 10 minutes, take 1 mL of the supernatant and inject it into the liquid phase Vials were subjected to LC-MS analysis.
[0031] The chromatographic conditions for LC-MS...
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