White tea manufacturing time determination method based on year white tea characteristic compound
A technology of characteristic compounds and identification methods, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of human subjectivity, tea identification, and obstacles to the development of white tea tea industry, etc., to achieve fast experimental operation and data analysis, and reduce requirements , the objective effect of the result
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[0025] Example 1:
[0026] Identification and discrimination of white tea years: through the identification of 8- CN -Ethyl-2-pyrrolidone-substituted epigallocatechin gallate content was determined, and then a linear discriminant model was established with the year, so as to identify and discriminate the white tea samples of unknown years by year.
[0027] Specific steps:
[0028] 1) Collect 18 Baihao Yinzhen white tea samples and 26 Bai Peony white tea samples of different years, grind them into powder and store them at -20℃.
[0029] 2) Determination of 8- CN - Content of ethyl-2-pyrrolidone-substituted epigallocatechin gallate:
[0030] Accurately weigh 0.1 g of white tea sample powder into a centrifuge tube, add 70% methanol aqueous solution and extract in a water bath at 70°C for 30 minutes, then centrifuge at 10,000 rpm for 10 minutes in a high-speed centrifuge, and take 1 mL of supernatant for liquid injection. vial for LC / MS analysis.
[0031] The chromatographi...
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