Qualitative detection method for pollen species and pollen volatile organic compounds

A technology of volatile organic compounds and identification methods, which is applied in the field of pollen analysis, can solve the problems of pollen sample damage and time-consuming, and achieve the effects of high accuracy, high-throughput detection and analysis, and improved efficiency

Pending Publication Date: 2018-07-27
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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Problems solved by technology

[0009] Aiming at the problems of long time consumption and damage to pollen samples in the identification process of pollen grains in the prior art, the present invention provides a qualitative analysis and detection method of pollen species and pollen volatile organic compounds

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  • Qualitative detection method for pollen species and pollen volatile organic compounds
  • Qualitative detection method for pollen species and pollen volatile organic compounds
  • Qualitative detection method for pollen species and pollen volatile organic compounds

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Experimental program
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Effect test

Embodiment 1

[0070] The detection method and principle of embodiment 1 pollen volatile organic compound

[0071] Using gas-phase ion mobility chromatography, the instrument can quickly detect volatile organic compounds in pollen after headspace sampling without vacuum and sample pretreatment, combined with related plug-ins and the built-in NIST gas phase retention index database and IMS According to the difference in retention time, ion migration time and ion peak intensity between different volatile organic compounds, different pollens were detected separately. It is characterized in that the method comprises the following steps:

[0072] Preparation steps of pollen volatile organic compound samples:

[0073] (1) Weigh 1.000g of pollen sample;

[0074] (2) Place directly into a 20mL headspace vial;

[0075] (3) The volatile gas after volatilization for 1 hour at normal temperature is used as a sample;

[0076] (4) The gas-phase ion mobility chromatograph separates and detects pollen v...

Embodiment 2

[0096] Implementation 2 Establishment of Standard Atlas of Volatile Organic Compounds in Pollen

[0097] The pollen of Humulus japonicus and Artemisia annua were used as the samples to be detected respectively, and the detection was carried out according to the method in Example 1, and the spectra were drawn. Among them, the standard spectrum of volatile organic compounds in Humulus pollen is as follows: figure 1 shown. The standard spectrum of volatile organic compounds in Artemisia annua pollen is as follows: figure 2 shown. figure 1 and figure 2 Middle: a. The ordinate represents the retention time of gas chromatography, and the abscissa represents the ion migration time; b. The background of the whole figure is black, and the vertical line at abscissa 1.0 is the RIP peak (reaction ion peak, after normalization); c .Each dot on either side of the RIP peak represents a VOC. The color represents the concentration of the substance, with white representing a lower concen...

Embodiment 3

[0098] Difference analysis between a small amount of pollen samples of embodiment 3

[0099] 1. Difference analysis among four kinds of autumn pollen samples

[0100] (1) Direct comparison of spectra: Visually observe the difference between sample spectra, such as image 3 shown. in, image 3 From left to right are ragweed-1, Artemisia grandis-1, Kochia-1, Kochia-2 and Humulus-1. It can be seen from the figure that there are great differences among different pollens, and Kochia There is a high degree of similarity between the two parallel samples.

[0101] (2) Difference comparison: select a spectrum as a reference, and subtract this spectrum from all the graphs. In the subtracted spectrum, the cleaner the background, the better the similarity with the reference, and the color on the graph The shade indicates the concentration of the substance, the darker the color, the higher the concentration than the reference, and the lighter the concentration of the substance is lower...

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Abstract

The invention provides a method for qualitatively analyzing pollen volatile organic compounds by using a gaseous migration chromatographic analysis technology so as to identify pollen species and purity. Volatile gas of a pollen sample is subjected to ionization detection of a gaseous transfer chromatography, three-dimensional qualification is carried out according to retention index, migration rate and strength of quasi-molecular ions, and a spectrogram is drawn. By qualitative analysis on features of different pollen species special volatile organic compounds, a characteristic gaseous transfer chromatogram map is drawn, a big database is established, and therefore, a qualitative identifying method for pollen species and quantitative calculating method for purity of the pollen are realized. By the method, the identifying time is short, the degree of automation is high, stability and reliability are high, the accuracy is also high, detection flux is large, protein ingredients of inspected pollen are not damaged, and meanwhile, on the basis of a big data analyzing technology, the method can be used for aerobiology monitoring, and is used for broadcasting pollen concentration.

Description

technical field [0001] The invention relates to the field of pollen analysis, in particular to a method for qualitatively detecting pollen species and pollen volatile organic compounds by using gas chromatography technology to analyze pollen volatile organic compounds. Background technique [0002] With the acceleration of industrialization and changes in (people's) lifestyles, the incidence of allergic reactions caused by inhaled allergens has increased significantly, seriously affecting the quality of life and work efficiency of the affected population, and increasing the economic burden of patients. Allergic diseases are common clinical diseases and frequently-occurring diseases, and are listed by the World Health Organization as one of the three major diseases that should be prevented and controlled in the 21st century. Allergic diseases may occur at all ages from newborns to the elderly, and have obvious genetic predispositions. Allergic diseases mainly include allergi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 周俊雄尹佳邢旭亚薛璐蒋健
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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