Water quality analyzing method and water quality analyzing device

A technology of water quality analysis and line fitting, applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of lack of threshold parameters for different background samples, short service life of filter membrane, cumbersome operation, etc., and achieve enhanced analysis accuracy and comparison Analytical ability, stable signal in the delay period, simple and efficient operation

Active Publication Date: 2013-11-06
FOCUSED PHOTONICS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the problems of microbial residues, cumbersome operation, short service life of the filter membrane and difficulty in realizing automation in the above-mentioned methods, it is difficult to effectively apply them in online instruments.
Therefore, the current detection equipment of the same kind still lacks effective online background enzyme interference countermeasures, and at the same time lacks a stable threshold parameter setting method for different background samples

Method used

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  • Water quality analyzing method and water quality analyzing device
  • Water quality analyzing method and water quality analyzing device
  • Water quality analyzing method and water quality analyzing device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051]A water quality analysis device, including a detection unit and an analysis unit, the detection unit includes a sampling module and a detection module, and the analysis unit includes a pre-judgment module, a fitting module and a background subtraction module;

[0052] 1. Detection unit

[0053] The detection unit is used to collect the sample to be tested and convert the detected reaction signal of the mixture of the sample to be tested and the culture medium into an electrical signal, and transmit it to the analysis unit, and the electrical signal corresponds to the detection time;

[0054] Preferably, the sampling module includes a peristaltic pump, a multi-channel directional valve, and a detection module. The channel is connected, and the detection module detects the signal of the sample to be tested and delivers it to the analysis unit.

[0055] The substrate can react with the microorganism-specific indicator and directly or indirectly send out a measurable signal...

Embodiment 2

[0097] A water quality analysis device, including a detection unit and an analysis unit, the detection unit includes a sampling module and a detection module, and the analysis unit includes a pre-judgment module, a fitting module and a background subtraction module;

[0098] The sample to be tested is a water sample containing fecal coliform bacteria as the target bacteria; the specific substrate is 4-methylumbelliferone-β-D-galactocuronide (MUGal) solution;

[0099] MUGal reacts specifically with β-D-galactosidase, a metabolic indicator produced by the growth of fecal coliform bacteria, and generates a fluorescent signal (excitation wavelength is 365nm, emission wavelength is 450nm).

[0100] The sampling module includes a peristaltic pump and a multi-channel directional valve, and the sample to be tested, the substrate and the measuring pool for containing the sample to be tested are respectively communicated with the multi-channel directional valve.

[0101] Because the del...

Embodiment 3

[0117] A water quality analysis device, different from the water quality analysis device in embodiment 2 is:

[0118] 1. The sample to be tested is a water sample containing Escherichia coli as the target bacterium; the specific substrate 4-methylumbelliferone-β-D glucuronide (MUG) solution;

[0119] MUG reacts specifically with the metabolic indicator β-D-glucuronidase produced by the growth of Escherichia coli to generate a fluorescent signal (excitation wavelength is 365nm, emission wavelength is 450nm).

[0120] 2. Because the delay period of Escherichia coli is usually about 2 hours, the time ratio M between the prediction period and the delay period is set to 1 / 3 in this embodiment, then the prediction module corresponding to the target bacteria set by the prediction module of this embodiment The time is 10min, and the ratio of it to the delay period is 1 / 12, which is less than M, then, before the fitting curve is obtained, the prediction module prompts that the substrat...

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Abstract

The invention relates to a water quality analyzing method, which includes the following steps: A, adding in a to-be-measured sample; B, setting the corresponding anticipated phase of an object bacterium, and the anticipated phase is in a lag phase; C, fitting the signals collected in the anticipated phase to achieve a fitted line; and D, starting measurement, obtaining the object curved based on the fitted line and a growth curve, and combining the object curved with the set threshold detection time so as to obtain the initial quantity of the object bacterium. The invention also provides a water quality analyzing device, and has the advantages that no special pretreatment is required, the water quality analyzing method and the water quality analyzing device are widely applicable and simple, and the background interference is effectively overcome.

Description

technical field [0001] The invention relates to a water quality analysis method and device, in particular to a water quality analysis method and device capable of subtracting background interference when no sample pretreatment is performed. Background technique [0002] Microbiological detection is an important part of environmental or food safety assessment. Among them, the total number of bacteria, coliform bacteria and pathogenic microorganisms are internationally recognized health detection indicators, which are important scientific basis for judging whether the environment or food is safe. Microbial detection can correctly evaluate the degree of microbial contamination of the environment or food, provide scientific basis for various health management work, and provide important scientific guidance for the targeted prevention and treatment of related diseases to ensure people's health; It is of great significance to the environment or food quality, to avoid economic loss...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 忻鼎承赵鹏
Owner FOCUSED PHOTONICS
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