Application of chloral hydrate in preparing metabolism regulator
A technology of chloral hydrate and regulators, which is applied in the direction of metabolic diseases, drug combinations, active ingredients of aldehydes, etc., and can solve problems such as rapid development
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[0014] 1. This experiment explores the mechanism of blood biochemical changes caused by chloral hydrate disturbing metabolic disorders in rats by combining metabolomics analysis platform.
[0015] 2. Materials and methods
[0016] 2.1 Experimental animals
[0017] Twenty-seven 7-week-old male rats, weighing 200-300 g, were provided by Shanghai Xipuer-Bikay Experimental Animal Co., Ltd. Experimental animal production license number: SCXK (Shanghai) 2013-0016, animals were kept in the barrier system of Nanjing Medical University Health Analysis and Testing Center (experimental animal use license number: SYXK (Su) 2015-0009), and used according to the experimental animal The 3R principle gives humane care.
[0018] 2.2 Instruments and reagents
[0019] Hitachi 7100 automatic blood biochemical instrument, UPLC Ultimate 3000system (Dionex) ultra-high performance liquid chromatography, Q-Exactive triple quadrupole tandem mass spectrometer, TraceFinder 3.1 software (Thermo Fisher ...
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