RAPD primers for identifying purity of seeds of watermelon variety Sumi No.6 and application thereof

A watermelon variety and seed technology, applied in DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of time-consuming, time-consuming, laborious, poor stability, etc., and achieve the effect of improving the quality control process

Active Publication Date: 2018-09-14
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a kind of RAPD primer that is used for identifying the seed purity of watermelon variety Sumi No. Quickly identify the seed purity of watermelon variety Sumi No. 6, the identification results are stable and reliable, and the operation is simple and convenient

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  • RAPD primers for identifying purity of seeds of watermelon variety Sumi No.6 and application thereof

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Embodiment 1

[0023] A kind of RAPD primer that is used to identify the purity of No. 6 seeds of watermelon variety Sumi, including primer JAASRP1059 and primer JAASCR1036, the nucleotide sequence of said primer JAASRP1059 is 5'-GTTGCCAGCC-3'; the nucleotide sequence of said primer JAASCR1036 The sequence is 5'-GTGACGTAGG-3'. Primers were synthesized by Qingke Biotechnology Co., Ltd.

[0024] Utilize above-mentioned primer to identify the method for No. 6 seed purity of watermelon variety Su Mi, comprise the steps:

[0025] (1) Extracting the genomic DNA of the watermelon variety Sumi 6;

[0026] The extraction method of genomic DNA is: the extraction method of genomic DNA is: get young tender blade 2g when watermelon variety Sumi No. 6 two leaves one heart, through liquid nitrogen grinding, with CTAB lysate (2%CTAB, 2M NaCl, 20mM EDTA , 100mM Tris-HCl (pH=8.0) and 0.2%-mercaptoethanol) into a 1.5mL centrifuge tube, take it out after 0.5-1h in a water bath at 65°C and cool it, add an equa...

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Abstract

The invention discloses RAPD primers for identifying the purity of seeds of a watermelon variety Sumi No.6. The RAPD primers comprise the primer JAASRP1059 and the primer JAASCR1036, and the sequenceof the primer JAASRP1059 is 5'-GTTGCCAGCC-3'; the sequence of the primer JAASCR1036 is 5'-GTGACGTAGG-3'. The purity of the seeds of Sumi No.6 can be identified by using the RAPD primer, and Sumi No.6DNA is extracted at first; by adopting the RAPD primers JAASRP1059 and JAASCR1036, PCR amplification is conducted with the genomic DNA of the seeds of the watermelon hybrid Sumi No.6 as a template; anamplified product is subjected to agarose gel electrophoresis detection. By comparing position differences of polymorphic amplified fragments formed by DNA fragment sequence differences and an electropherogram, the genetic purity of Sumi No.6 can be identified, an identification result is quick, stable and reliable, and the operation is simple and convenient.

Description

technical field [0001] The invention relates to a RAPD primer for identifying the seed purity of a watermelon variety and an application thereof, in particular to an RAPD primer for identifying the seed purity of a watermelon variety Sumi No. 6 and an application thereof, belonging to the technical field of crop breeding. Background technique [0002] Watermelon is an annual herb of Cucurbitaceae Cucurbitaceae and is an important worldwide horticultural crop. my country is a big producer of watermelon, and its planting area and output both rank first in the world. At present, new watermelon varieties in the domestic market have achieved 100% hybrids. The quality of watermelon seeds is closely related to the development of the watermelon industry, and also directly affects the economic benefits of melon farmers. However, in the process of watermelon hybrid seed production, due to reasons such as insects or wind pollination, the seeds of the female parent line are often mixed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 刘广羊杏平徐锦华张曼姚协丰侯茜任润生朱凌丽
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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