Fast reproduction method for tissue culture of mussaenda hybrida cv.Alicia

Abstract

The invention discloses a fast reproduction method for tissue culture of mussaenda hybrida cv.Alicia. According to the method, stems which are provided with leaf buds and generated in the current yearby stock plants with excellent phenotypic traits are adopted as explants, the explants are cleaned, sterilized and then inoculated into an adventitious bud inducing culture medium for culture, adventitious buds are transplanted into a proliferation culture medium for proliferation culture for 35-40 days after adventitious bud inducing culture which lasts for 20-30 days, and after that, the proliferation rate can be increased by 4-5 times. Finally, the proliferated buds are inoculated into root and seedling boosting culture mediums separately for inducing culture of robust root and seedlings,and after the 60-day-long robust seedlings cultivation, the rooting rate can reach 100%. The number of seedlings of mussaenda hybrida cv.Alicia within a cycle of 120-130 days can be increased by 4-5 times; compared with traditional cutting propagation, the method has the advantages that the propagation time can be shortened by half or above, limitation of conditions in the aspects such as seasons,climate and land usage is omitted, the quality of plantlets is high, the number of the plantlets is sufficient, and the demand of the market for garden landscaping and afforestation can be met quickly.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for rapid propagation of golden flower tissue culture. Background technique [0002] Pink leaf golden flower, scientific name Mussaenda hybrida cv.Alicia, alias pink calyx flower, pink paper fan, native to tropical Africa and Asia, belonging to Rubiaceae. The plant height is 1 to 2 meters, strong in nature, high temperature-loving, and drought-tolerant. The sepals are hypertrophic, and the whole plant is pink when in full bloom, very eye-catching, and the flowering period is from summer to autumn and winter. For many years, it has only been cultivated in the wild or in tropical and subtropical botanical gardens. With the rise of landscaping in recent years, Golden Leaf Flower has green leaves, fast growth, beautiful flowers, long flowering period and strong adaptability; it is suitable for potted plants, planting in flower tanks, The various characteristic...

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Problems solved by technology

[0003] The object of the present invention is to provide a method for rapid propagation of T. aurantia tissue culture, which fills the gap in the research on tissue culture of T. argentina, solves the problems of low propagation rate, long propagation cycle and high cost of traditional T. aurantia cuttings, and realizes rapid industrialized breeding of T. argentina

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