Cell density control instrument and its method and application

Abstract

The invention discloses a cell density control instrument and its method and application. The cell density control instrument includes a laser light source, a collimator lens, a screen and a cell culture platform arranged sequentially along the laser light path. The temperature control device can sense the temperature of the cell culture platform, and turn off the laser light source when the temperature reaches the set temperature; the cell culture platform is equipped with grooves and fixtures for accommodating traditional cell culture plates or culture dishes, and the laser light source is projected onto the collimating lens Finally, the collimator lens emits the laser in parallel, and after the parallel laser passes through the screen, the laser is blocked into a large number of parallel light bands with the same interval and projected on the culture plate. Using the principle that high temperature causes cell damage and death, the cell density is controlled at a set level, which provides a more reliable means and basis for further clarifying the cell proliferation ability and improving the success rate of cell plasmid transfection.

Description

technical field [0001] The invention relates to the technical fields of cell phenotype, cell biology and photothermometry, and further relates to a cell density control instrument and its method and application. Background technique [0002] The cell proliferation test (MTT method) can monitor the changes in the proliferation rate of adherent cells, and is widely used in the field of basic research on cell activity experiments. The enhancement of cell proliferation ability is often a characteristic performance in the process of tumor cell development. Interfering with cell proliferation ability is beneficial to control the growth of tumors and slow down the development and deterioration of tumor patients. Therefore, cell proliferation experiment is an important means in the field of medical basic research, and has extensive practical value. [0003] The purpose of the plasmid transfection experiment is to transfer the plasmid carrying the target gene into the host cell and ...

AI Technical Summary

Problems solved by technology

This requires high experimental skills for the operator. Because the traditional counting method is easily affected by human factors, the uneven distribution of cells will cause large deviations in the results, and the repeatability of the experiment is poor.

[0008] (2) The traditional cell counting method is time-consuming, and the process of repeated digestion, pipetting, and centrifugation of the cells will cause greater damage to the cells, especially for primary cultured cells, which may lead to a large number of cell death

However, the transfection efficiency of the cells after plasmid transient transfer is increased by one passage, which seriously affects the observation and research of the effect on the target gene.

[0009] Although the smart cell counters currently on the market can reduce subjective counting errors, they also have technical defects: they only count cells within a selected small field of view, and there may still be errors if multiple points are selected for counting and time-consuming; The ability to identify residues in some solutions is limited, which affects the counting results; special cell slides are consumables, which increase the cost of cell experiments

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