System and method capable of preventing microbiological contamination during inoculation of microbial fermentation

A microbial fermentation and inoculation bottle technology, applied in the field of microbial fermentation, can solve the problems of easy-to-contaminate bacteria and achieve the effect of avoiding bacterial contamination

Inactive Publication Date: 2018-10-12
张斌行
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention provides a system and method for microbial fermentation to avoid bacterial contamination dur

Method used

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  • System and method capable of preventing microbiological contamination during inoculation of microbial fermentation
  • System and method capable of preventing microbiological contamination during inoculation of microbial fermentation

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Embodiment Construction

[0026] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following will describe in detail with reference to the drawings and specific embodiments.

[0027] On the one hand, the present invention provides a kind of microbial fermentation avoids the system of bacterial infection when inoculation, such as figure 1 and figure 2 As shown, including inoculation bottle 1 and fermenter 2, wherein:

[0028] The mouth of the inoculation bottle 1 is provided with a rubber stopper 1-1, the middle part of the rubber stopper 1-1 is provided with a first through hole 1-2, and the outer side of the bottle mouth is provided with a gland 1-3, and the gland 1-3 is provided with a rubber plug. The plug 1-1 is pressed tightly in the bottle mouth, and the middle part of the gland 1-3 is provided with a second through hole 1-4 concentric with the first through hole 1-2;

[0029] The fermenter 2 comprises a tank body 2-1...

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Abstract

The invention relates to a system and a method capable of preventing microbiological contamination during inoculation of microbial fermentation, belonging to the technical field of microbial fermentation. The system comprises an inoculating bottle and a fermentation tank, wherein a rubber plug is arranged in the bottleneck of the inoculating bottle, a first through hole is formed in the middle ofthe rubber plug, a gland is arranged at the outer side of the bottleneck, the gland tightly presses the rubber plug into the bottleneck, and a second through hole concentric with the first through hole is arranged in the middle of the gland; the fermentation tank comprises a tank body and a sealing cover arranged on the tank body, a material supplementing pipe is arranged on the sealing cover, a material supplementing valve is arranged on the material supplementing pipe, a first short joint and a second short joint connected in sequence are arranged between the material supplementing valve andthe sealing cover, an inoculating valve is arranged between the first short joint and the second short joint, a latex hose sleeves the tail end of the second short joint, an insertion head made of astainless steel tube sleeves the tail end of the latex hose, the insertion head made of the stainless steel tube is inserted in the first through hole and the second through hole, and an emptying valve is further arranged on the sealing cover. With the system and the method provided by the invention, the problem that microbiological contamination easily occurs during inoculation of microbial fermentation can be solved.

Description

technical field [0001] The invention relates to a system and method for microbial fermentation to avoid bacterial contamination during inoculation, and belongs to the technical field of microbial fermentation. Background technique [0002] Since the 1990s, my country has carried out extensive research on biotechnology, and has achieved a number of breakthrough results. A variety of new substances and products have been produced through microbial fermentation, which are used in people's work and life. [0003] After studying the physiological characteristics of microorganisms, the inventor carried out a number of experimental activities on microbial fermentation, such as: bacteria fermented in glycerin to produce 1,3 propanediol, enzyme bacteria fermented in starch milk to produce glucose, etc., all achieved good results. During the experiment, since the aseptic room, ultra-clean platform, shaker and other parts were strictly sterilized, the phenomenon of seed contamination r...

Claims

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Application Information

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IPC IPC(8): C12M1/26C12M1/24C12M1/02C12M1/00
CPCC12M23/08C12M23/38C12M27/02C12M33/04
Inventor 张斌行
Owner 张斌行
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