Method for avoiding dormancy of seeds of Paris polyphylla var.chinensis by using exogenous growth regulating agent

A growth regulator, exogenous technology, applied in the direction of plant growth regulators, plant growth regulators, biocides, etc., can solve problems such as germination rate needs to be improved

Pending Publication Date: 2018-10-16
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the germination rate of th

Method used

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  • Method for avoiding dormancy of seeds of Paris polyphylla var.chinensis by using exogenous growth regulating agent
  • Method for avoiding dormancy of seeds of Paris polyphylla var.chinensis by using exogenous growth regulating agent
  • Method for avoiding dormancy of seeds of Paris polyphylla var.chinensis by using exogenous growth regulating agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The seeds of Huazhonglou were taken, peeled manually, then mixed with wet sand (humidity 10%), and stratified at low temperature for 50 days. Take out the above-mentioned low-temperature stratified seeds for 50 days, sieve away the sand, rinse them with clean water, soak the seeds in different concentrations of exogenous growth regulator solutions for 48 hours, and then treat them with 7% sodium hypochlorite solution for 30 minutes, take them out with clean water Wash off the residual liquid, then disinfect with 70% alcohol for 1 min, use double-layer absorbent cotton gauze as the germination bed, and germinate in an artificial climate box at a temperature of 25°C and in darkness for 90 days. Water treatment was used as blank control.

[0026] The above exogenous growth regulator is any one or more of GA3, 6-BA, 2,4-D and KMnO4, the concentration of GA3 is 500mg / L or 800mg / L, and the concentration of 6-BA is 10mg / L or 50mg / L, the concentration of 2,4-D is 0.1mg / L, and...

Embodiment 2

[0053] To configure a compound regulator, take a certain amount of GA3, KMnO4 and 6-BA, add water, and configure a compound regulator containing 500mg / LGA3, 0.3% KMnO4 and 10mg / L 6-BA in the form of a solution.

[0054] The seeds of Huazhonglou were taken, peeled manually, then mixed with wet sand (humidity 10%), and stratified at low temperature for 50 days. Take out the above-mentioned low-temperature stratified seeds for 50 days, sieve away the sand, rinse them with clean water, soak the seeds in the compound conditioner for 48 hours, and then treat them with 7% sodium hypochlorite solution for 30 minutes, take them out and wash off the residual liquid with clean water, Then sterilize with 70% alcohol for 1 min, use double-layer absorbent cotton gauze as the germination bed, and germinate in an artificial climate box for 90 days at a temperature of 25°C and in the dark.

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Abstract

The invention relates to the technical field of planting, and in particular relates to a method for avoiding dormancy of seeds of Paris polyphylla var.chinensis by using an exogenous growth regulatingagent. The method comprises the following steps: immersing the seeds into an exogenous growth regulating agent solution for 48 h, performing cleaning, performing disinfection, using double-layered degreasing cotton gauze as a bud bed, and performing germination for 90 days in a manual climatic box at temperature of 25 DEG C under dark condition. According to the method provided by the invention,the germination effects of the seeds of the Paris polyphylla var.chinensis are as follows: GA3 > 2,4-D > 6-BA > KMnO4 > CK > alpha-NAA, and when a best combination of 500 mg/L GA3, 0.3% KMnO4 and 10 mg/L 6-BA is used to process the seeds of the Paris polyphylla var.chinensis, the germination rate and germination force of the seeds can be significantly improved, the germination rate of the seeds can reach 91.33%.

Description

technical field [0001] The invention relates to the field of planting technology, in particular to a method for breaking the dormancy of seeds of huazhonglou with an exogenous growth regulator. Background technique [0002] Huazhonglou [Latin: Paris polyphylla Smith var.chinensis (Franch.) Hara] is a plant of the genus Paris polyphylla, which is a kind of Chinese medicinal material. It is a perennial herb, 30-100cm high. Stems erect. The leaves are 5-8 in whorls on the top of the stem. The leaves are oblong-lanceolate, obovate-lanceolate or oblanceolate, 7-17cm long and 2.5-5cm wide. The pedicel emerges from the top of the stem, usually longer than the leaves, with a terminal flower, 4-6 sepals, leaf-like, green, 3-7cm long; perianth thin linear, yellow or yellow-green, 1-1.5mm wide, 1.5mm long Sepals 1 / 3 to nearly as long; stamens 8-10, anthers 1.2-2cm long. The capsule is spherical, and the seeds have red fleshy arils. The flowering period is from May to July, and the...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01N59/16A01N43/90A01N45/00A01N43/12A01P21/00
CPCA01C1/00A01N43/12A01N43/90A01N45/00A01N59/16A01N2300/00
Inventor 张春椿李石清张水利范慧艳
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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