The invention discloses a daffodil seed stem preservation method. The method comprises the following aspects: (1) processing a seed stem, choosing a high-quality corm, slightly removing an outer filmof the seed stem with hands, and then sterilizing; (2) coating a film, adding chitosan and penicillin into a fermentation solution, stirring, mixing, preserving heat to obtain a film coating solution,baking the sterilized seed stem at the low temperature, and soaking in the film coating solution; (3) irradiating at the low temperature, putting the film coated seed stem into a variable temperaturechamber to process at the variable temperatures, and irradiating through ultraviolet light under the low temperature condition, (4) preserving, levelling fine sand at the bottom part of a sand pit, spreading screened fine earth, spreading the processed seed stem on the screened fine earth, respectively spreading the screened fine earth and the fine sand, horizontally placing a bamboo tube on themiddle layer of the seed stem, spreading an ice bag at the upper part of a sand pile, and using an old quilt and a plastic film to wrap the sand pile; (5) accelerating germination: airing the preserved seed stem in the sun, soaking in an aspirin solution, accelerating germination at the constant temperature, and cultivating the seed stem after accelerating germination.