Process for producing dark tea through inoculation method
A process and technology of dark tea, applied in the field of microorganisms and food biology, can solve the problems of unstable quality of dark tea products, uncontrollable number of golden flowers, etc., and achieve simple and feasible inoculation process, significant number of flowers, and increased number of flowers Effect
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Embodiment 1
[0058] Example 1 Separation and purification of strains
[0059] The steps for the separation and purification of the superior "Golden Flower" bacteria of black tea are as follows:
[0060] (1) Select tea samples
[0061] Select Hunan Anhua Tea Factory The Fuzhuan tea products of different years in the tea in the wooden warehouse and the mainstream Fuzhuan tea products produced and sold in Yiyang City, Hunan Province are used as the original strain isolation materials. The tea samples of the above selected products meet the requirements of GH / T1070 for tea packaging, meet the requirements of GH / T1071 for tea storage, meet the limit of contaminants in food in GB2762-2012, and meet the limit of maximum pesticide residue in GB2763 / GB26130 , To meet the relevant standards and regulations of GB / T9833.2-2013 for "Pressed Tea"; at the same time, the selected tea samples meet: the packaging is free of damage and stains; the shape of the tea is flat, and the flower inside the tea brick is e...
Embodiment 2
[0077] Example 2 Breeding of strains
[0078] Breeding steps a. According to the characteristics of the bacteria contained in the tea during fermentation and the growth of golden flowers, the strains are selected for the growth of "golden flowers" in tea
[0079] Breeding ideas: Under the same culture conditions, the same batch of black hair tea raw materials from the same producing area were selected for the fermentation test of strain inoculation. If the strain can meet the requirements of the quality of the tea for the characteristics of the fermenting bacteria and the growth degree of the "golden flower" for the strain, then after the inoculation of the strain, the strain can grow rapidly in the tea product and within a certain period of time The fermented tea can reach a certain number of bacteria, which can grow into dominant bacteria and inhibit the growth of miscellaneous bacteria; and its good characteristics are reflected in the appearance and quality of the final tea. O...
Embodiment 3
[0098] Example 3 Identification of bacterial species
[0099] The morphological, physiological and biochemical characteristics, and molecular biological characteristics of EurotiumcristatumNHRI-BC-1.5.1 obtained in Example 2 above were identified, as follows:
[0100] A. Morphological characteristics of bacteria
[0101] Take the strain numbered NHRI-BC-1.5.1, inoculate the strain on the PDA solid plate medium by the streak culture method, and cultivate it in a constant temperature incubator at 28°C for 15 days. Pick the medium when the bacterial morphology is observed The mycelium on the colony was observed under an optical microscope with a magnification of 10 times the objective lens and 16 times the eyepiece.
[0102] Observation results showed that the bacteria were filamentous, separated and light yellow.
[0103] B. Colony morphological characteristics
[0104] Take the strain numbered NHRI-BC-1.5.1, use spot culture method to inoculate the strain on the PDA solid plate medium, a...
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