Lead cheating type immune complex with excellent effect and preparation method thereof

Abstract

The invention discloses a lead cheating type immune complex with excellent effect. The lead cheating type immune complex with excellent effect is selected from any of complexes, such as a complex which is formed by binding lead ions with an immune complex; or, a complex which is formed by binding lead ions with carrier protein and then binding with an antibody which is specifically bound with thecarrier protein; or, a complex which is formed by binding lead ions with immune globulin and then binding with the carrier protein. The invention also discloses a preparation method of the lead cheating type immune complex with excellent effect. The preparation method comprises the following steps of S1, preparing a chelating agent solution; S2, preparing a carrier protein solution; S3, stirring and staying overnight; S4, dialyzing; S5, adding the lead ions; S6, recycling waste liquid; S7, specifically binding. The lead cheating type immune complex with excellent effect has the characteristicsthat the application range is broader, the cost is reduced, the dialysis rate is increased, the preparation cycle is shortened, and the energy-saving and environment-friendly effects are realized; the chemical pollution is avoided; the market competitiveness is larger.

Description

technical field

[0001] The invention relates to the field of chelates, in particular to a lead chelating immune complex with excellent effects and a preparation method thereof. Background technique

[0002] Chelates are widely used in mineral flotation process, hydrometallurgy, extraction and separation of metal elements, catalytic synthesis of substances, softening of water, electroplating process, pharmaceutical industry, dyeing process, etc.; the existing lead chelate The preparation method of the compatible immune complex is not perfect, and the scope of application is not wide enough. It is inconvenient to complete the preparation process of the target in a small laboratory, and the number of dialysis will prolong the preparation period of the target to a certain extent. There is no clear treatment of ions and other wastes in the waste liquid, and the discharge of ions and other wastes in the waste liquid will cause chemical pollution if they are not treated up to the s...

Examples

preparation example Construction

[0033] A preparation method of the lead chelating immune complex as claimed in claim 1, comprising the following steps:

[0034] S1: First prepare the chelating agent solution, and then dissolve the chelating agent in the solvent to obtain the chelating agent solution;

[0035] S2: Take an appropriate amount of carrier protein, and add the carrier protein into the borate buffer solution to prepare the carrier protein solution;

[0036] S3: Add the prepared chelating agent solution to the carrier protein solution, place it in a constant temperature water bath and stir evenly, and keep the constant temperature water bath for 24 hours to obtain the mixed solution;

[0037] S4: Take the dialysis bag and add EDTA-NaHCO3 solution into the dialysis bag to boil, fix the dialysis bag in the middle of the water tank, and add enough ddH2O to the water tank, the volume ratio of the ddH2O to the solution in the dialysis bag is 91:1, repeat this step twice, put the mixture in S3 into a dia...

technical field

[0001] The invention relates to the field of chelates, in particular to a lead chelating immune complex with excellent effects and a preparation method thereof. Background technique

[0002] Chelates are widely used in mineral flotation process, hydrometallurgy, extraction and separation of metal elements, catalytic synthesis of substances, softening of water, electroplating process, pharmaceutical industry, dyeing process, etc.; the existing lead chelate The preparation method of the compatible immune complex is not perfect, and the scope of application is not wide enough. It is inconvenient to complete the preparation process of the target in a small laboratory, and the number of dialysis will prolong the preparation period of the target to a certain extent. There is no clear treatment of ions and other wastes in the waste liquid, and the discharge of ions and other wastes in the waste liquid will cause chemical pollution if they are not treated up to the s...

Examples

preparation example Construction

[0033] A preparation method of the lead chelating immune complex as claimed in claim 1, comprising the following steps:

[0034] S1: First prepare the chelating agent solution, and then dissolve the chelating agent in the solvent to obtain the chelating agent solution;

[0035] S2: Take an appropriate amount of carrier protein, and add the carrier protein into the borate buffer solution to prepare the carrier protein solution;

[0036] S3: Add the prepared chelating agent solution to the carrier protein solution, place it in a constant temperature water bath and stir evenly, and keep the constant temperature water bath for 24 hours to obtain the mixed solution;

[0037] S4: Take the dialysis bag and add EDTA-NaHCO3 solution into the dialysis bag to boil, fix the dialysis bag in the middle of the water tank, and add enough ddH2O to the water tank, the volume ratio of the ddH2O to the solution in the dialysis bag is 91:1, repeat this step twice, put the mixture in S3 into a dia...

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