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Method for detecting glyphosate and metabolite of glyphosate in sugarcanes

A technology for the detection of glyphosate and glyphosate, which is applied in the field of rapid detection of glyphosate and its metabolites in sugarcane by high performance liquid chromatography tandem mass spectrometry, can solve the problems of long time consumption and many false positives, and achieve simple operation and elimination False positives, the effect of overcoming sugar interference

Active Publication Date: 2018-10-19
广东省科学院生物与医学工程研究所
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Problems solved by technology

However, this method still needs a derivative reaction, which takes a long time and has many false positives, so it still cannot meet the requirements

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  • Method for detecting glyphosate and metabolite of glyphosate in sugarcanes
  • Method for detecting glyphosate and metabolite of glyphosate in sugarcanes
  • Method for detecting glyphosate and metabolite of glyphosate in sugarcanes

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[0040] The present invention will be further described by examples below, and these descriptions are not intended to further limit the content of the present invention. Those skilled in the art should understand that equivalent replacements or corresponding improvements made to the content of the present invention still fall within the protection scope of the present invention.

[0041] A high performance liquid chromatography tandem mass spectrometry detection method for glyphosate and its metabolites in sugarcane, the specific implementation steps are as follows.

[0042] (1) Preparation of standard solution and detection by high performance liquid chromatography tandem mass spectrometry

[0043] Accurately weigh glyphosate (PMG) and 10 mg of glyphosate metabolite aminomethyl phosphate (AMPA) respectively in equal amounts and place them in a 100 mL volumetric flask, and dissolve them with acetonitrile water to the mark and use them as a mixed standard stock solution; This m...

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Abstract

The invention relates to quantitative detection of glyphosate and metabolite of glyphosate in sugarcanes, and provides a HPLC-MS method for detecting glyphosate and metabolite of glyphosate in sugarcanes. The method comprises following steps: (1) preparing standard solutions, and carrying out HPLC-MS detection to obtain standard spectrum diagrams of mixed standard solutions with different concentrations; (2) based on the standard spectrum diagrams obtained in the step (1), according to glyphosate and metabolite of glyphosate, individually manufacturing curves representing the relationship between concentrations and chromatographic peak areas; (3) preprocessing a sugarcane sample, and detecting the sample by HPLC-MS; (4) determining the contents of glyphosate and metabolite of glyphosate inthe sample: based on the retention time in the sample spectrum obtained in the step (3), determining the kind of glyphosate and metabolite of glyphosate, comparing the chromatographic peak areas of glyphosate and metabolite of glyphosate with the standard curve obtained in the step (2), and determining the contents of glyphosate and metabolite of glyphosate by an insertion method. The provided method has the characteristics of simple preprocessing process, quick detection speed, accurate detection result, and high sensitivity.

Description

technical field [0001] The invention relates to the quantitative detection of glyphosate and its metabolites in sugarcane, in particular to the rapid detection method of glyphosate and its metabolites in sugarcane by high performance liquid chromatography tandem mass spectrometry. Background technique [0002] Sugarcane is one of the most important sugar crops in the world. According to the statistics of the Ministry of Agriculture, the world's total sugar production in 2008-2009 was 150 million tons, and the sucrose production was 117.5 million tons, accounting for 78.33% of the total sugar production. my country is the world's third largest sugar producer and sugar consumer, with both sugar production and consumption accounting for about 7% of the world's total sugar production and consumption. Sugarcane is the most important sugar crop in my country, with a planting area of ​​1,125,100 hectares in Guangxi, accounting for about 70% of the country. In the process of sugarc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045
Inventor 甄振鹏高裕锋黄敏兴李家威陈其钊杨李胜刘志鹏庞扬海王小鹏余构彬郭剑雄
Owner 广东省科学院生物与医学工程研究所
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