Acute different liver damage inducer and preparation method and application thereof

An inducer and differential technology, applied in pharmaceutical formulations, organic active ingredients, medical preparations containing active ingredients, etc., can solve acute differential liver injury, immune dual factor damage, liver damage and liver function damage The same model cannot objectively reflect problems such as liver injury, and achieves the effect of low condition requirements, short modeling cycle, and high modeling success rate

Inactive Publication Date: 2018-10-23
AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the complexity of liver injury and the diversity of liver function damage make the same model unable to reflect the liver injury comprehensively and objectively in essence, and the prior art does not me

Method used

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  • Acute different liver damage inducer and preparation method and application thereof
  • Acute different liver damage inducer and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 A preparation method of an acute differential liver injury inducer

[0038]1) Dissolve 800 mg of D-galactosamine and 10 μg of lipopolysaccharide in 10 mL of medical saline to obtain a solution;

[0039] 2) Filter the solution with a 0.22 μm bacteria filter to obtain an acute differential liver injury inducer, and store it at -20°C.

Embodiment 2

[0040] Embodiment 2 A kind of modeling method of acute differential liver injury model mouse

[0041] 1) Take 8 clean same-sex Kunming mice with a body weight of 25±2g and a growth week of 5 as a model group, fasting for 12 hours, and set aside;

[0042] 2) Inject the acute differential liver injury inducer prepared in Example 1 into the abdominal cavity of the mice in the model group for one injection. During the injection, select the injection needle at a place about 0.5 cm away from the linea alba in the lower abdomen of the mouse. Insert subcutaneously, advance 3-5cm along the subcutaneously, and then insert the needle into the abdominal cavity at an angle of about 30° with the abdomen of the mouse. , the needle after entering the abdominal cavity does not exceed 1cm, the head of the mouse is lowered during injection, and the tail is slightly raised.

[0043] 3) 12 to 48 hours after the injection of the inducer, observe the activity response of the mice; and kill the abov...

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Abstract

The invention provides an acute different liver damage inducer and a preparation method and application thereof and belongs to the field of animal model making. The effective components of the acute different liver damage inducer comprise D-galactosamine and lipopolysaccharide and is characterized in that the concentration of the D-galactosamine in the inducer is 50-120mg/mL; the concentration ofthe lipopolysaccharide in the inducer is 0.1-10 microgram/mL; the solvent of the inducer is normal saline. The application of the inducer includes: applying the acute different liver damage inducer tothe abdominal cavity of a clean mouse so as to obtain an acute different liver damage model mouse after 12-48 hours. The acute different liver damage inducer is high in application success rate, lowin condition requirements, simple in method, short in model making cycle, good in repeatability, capable of achieving evident liver damage change and capable of satisfying the requirements of liver tests.

Description

technical field [0001] The invention relates to the technical field of animal model modeling, in particular to an acute differential liver injury inducer and its preparation method and application. Background technique [0002] Liver injury is a clinical syndrome of liver function damage caused by a variety of etiologies, characterized by a large number of liver cell degeneration, necrosis, and inflammatory cell infiltration in a short period of time, with high mortality. Liver cell necrosis is a common pathological feature of various types of liver injury, including: hepatic cell necrosis (necrosis), programmed necrosis (necroptosis), apoptosis (apoptosis) and autophagy (autophagyd). Liver disease progression, that is, the survival of liver cells The state is related to a variety of factors. Under the action of various pathogenic factors, liver cells maintain the stability of the intracellular environment and promote cell survival by initiating various protective mechanisms...

Claims

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Application Information

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IPC IPC(8): A61K31/739A61K31/7008
CPCA61K31/739A61K31/7008
Inventor 何毅怀沈访汪杰陈贵梅唐永静陈娉婷
Owner AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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