Method for detecting, locating and monitoring seepage and leakage of hydraulic structures

A technology of hydraulic engineering and comprehensive methods, applied in the fields of biochemical equipment and methods, and the determination/inspection of microorganisms, which can solve problems such as biological toxicity, hidden dangers, and signal reduction over time.

Inactive Publication Date: 2018-10-23
HUNAN ZONSEN PEPLIB BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some of these drawbacks include safety concerns, generation of radioactive waste, biological toxicity, and signal degradation over time due to radioactive decay
In addition, the production, transportation, use and disposal costs required for radioisotopes are also an issue

Method used

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  • Method for detecting, locating and monitoring seepage and leakage of hydraulic structures
  • Method for detecting, locating and monitoring seepage and leakage of hydraulic structures
  • Method for detecting, locating and monitoring seepage and leakage of hydraulic structures

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Embodiment Construction

[0021] Unless defined otherwise, all technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. All publications and patents mentioned in this specification are incorporated herein by reference.

[0022] Embodiments of the invention relate to methods for highly sensitive tracking of fluid flow using sequence-specific nucleic acids as tracers. In one aspect, the present invention involves the use of sequence-specific nucleic acids as tracers to allow for a significant increase in detection sensitivity. For example, the present invention provides an improved tracking method whereby even single molecules of DNA in a sample can be detected by enzymatic amplification methods such as polymerase chain reaction (PCR).

[0023] The terms "DNA", "probe", "tracer", "nucleic acid", "vector", "plasmid", "enzyme", "liquid", "polymerase chain reaction (PCR) ", "seepage", "leakag...

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PUM

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Abstract

The disclosure relates to an improved method for detecting, locating and monitoring fluid seepage and leakage from a hydraulic work with superior sensitivity. The method includes using a DNA sequenceas the probe to trace the fluid seepage and leakage from a hydraulic work. The probe can be captured and then amplified more than a millionfold by an enzymatic method such as the polymerase chain reaction (PCR) to give a detection signal. Even a single molecule of the DNA probe can be detected by an enzymatic amplification, thus to give superior sensitivity. The improved detection method is applicable to detecting, locating and monitoring fluid seepage and leakage from hydraulic works, the improved method can also be used, for example, to trace the groundwater flow, underground water flow andother liquid flow. Other related methods are also described.

Description

technical field [0001] The invention relates to an improved method for detecting, locating and monitoring liquid seepage and leakage in water conservancy projects with high sensitivity. The method includes: using the DNA sequence as a probe to track the seepage and leakage of the liquid in the water conservancy project. The probe is captured and then amplified more than 1 million times by an enzymatic method such as polymerase chain reaction (PCR) to generate a detection signal. Through enzymatic amplification, even single-molecule DNA probes can be detected, enabling highly sensitive detection. The improved method is applicable to the detection, location and monitoring of liquid seepage and leakage in hydraulic engineering. The improved method can also be used, for example, to track groundwater, groundwater runoff, and the flow of other liquids. Background technique [0002] Water conservancy projects such as dams and reservoirs are important assets that benefit modern s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6851
CPCC12Q1/68C12Q2563/185C12Q1/6876
Inventor 王珠银申明霞蒋林华
Owner HUNAN ZONSEN PEPLIB BIOTECH CO LTD
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