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Rapid propagation method for malus halliana

A fast technology, applied in the field of rapid propagation of Begonia, can solve the problems of long cultivation time, low survival rate, and large individual differences of Begonia, and achieves short cultivation period, high survival rate, and easy to solve problems. The effect of low cutting survival rate

Inactive Publication Date: 2018-11-02
句容市茂润苗木有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Chuisi Begonia is a deciduous tree belonging to the Rosaceae apple genus. It is an important ornamental tree species. It is cultivated in various places. Its taste is light, bitter, and flat in nature. Its flowers are used as medicine. It has the effect of regulating menstruation and activating blood circulation. For bruises, fractures and other diseases, the dying crabapple also has certain edible functions. Its fruit is sweet and sour, and can be made into candied fruit. It is used in a large amount in food and health products, especially in recent years. The demand for Chuisi Begonia is also increasing. Usually, the methods of breeding Chuisi Begonia mainly include ramets, grafting, layering, and cuttings. The ramets germinate from the rhizosphere of the mother plant in March in spring. The sprouted seedlings are gently separated for transplanting, and the method of branching and grafting is used for breeding, the survival rate is low, and the number of mother plants and rootstocks is required, and after transplanting or grafting, individual differences Larger, low survival rate, at the same time, longer cultivation time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A kind of rapid breeding method of crabapple, comprising the following steps:

[0017] (1) Cut off the terminal buds of 3-4-year-old robust branches, soak the cut terminal buds in alum for 10 minutes, then rinse them with clean water, dry them naturally in the shade, then soak them in hydrogen peroxide for 2 minutes, 75% medical Rinse with alcohol for 20 s, and finally rinse with double distilled water; place the terminal buds after rinsing on the primary culture medium, and after culturing for 15 days, obtain the terminal bud callus of Begonia chinensis; the primary medium is: MS As the basic medium, add 0.1 mg / L 6-benzylpurine, 0.1 mg / L zeatin, 0.5 g / L gibberellin, 0.5 mg / L ammonia water, 30 g / L glucose and 3.0 g / L agar, adjust The pH value of the induction medium was 5.8-6.2; the culture conditions were: the culture temperature was 25 °C, the light intensity was 2000 Lux, and the light time was 10 h / d.

[0018] (2) Place the callus of the top buds of Begonia chinens...

Embodiment 2

[0022] A kind of rapid breeding method of crabapple, comprising the following steps:

[0023] (1) Cut off the terminal buds of 3-4-year-old robust branches, soak the cut terminal buds in alum for 10 minutes, then rinse with clean water, dry the rinsed terminal buds naturally, and then soak them in hydrogen peroxide for 3 minutes, 75% medical use Rinse with alcohol for 20 s, and finally rinse with double distilled water; place the terminal buds after rinsing on the primary culture medium, and after culturing for 20 days, obtain the terminal bud callus of Begonia chinensis; the primary medium is: MS As the basic medium, add 2.0 mg / L 6-benzylpurine, 1.0 mg / L zeatin, 3.0 g / L gibberellin, 3.0 mg / L ammonia water, 50 g / L glucose and 5.0 g / L agar, adjust The pH value of the induction medium was 5.8-6.2; the culture conditions were as follows: the culture temperature was 30 °C, the light intensity was 3000 Lux, and the light time was 12 h / d.

[0024] (2) Place the callus of the top bu...

Embodiment 3

[0028] A kind of rapid breeding method of crabapple, comprising the following steps:

[0029] (1) Cut off the terminal buds of 3-4-year-old robust branches, soak the cut terminal buds in alum for 10 minutes, then rinse them with clean water, dry them naturally in the shade, then soak them in hydrogen peroxide for 2.5 minutes, 75% medical Rinse with alcohol for 20 s, and finally rinse with double distilled water; place the terminal buds after rinsing on the primary culture medium, and after culturing for 17 days, obtain the terminal bud callus of Begonia chinensis; the primary medium is: MS As the basic medium, add 1.0 mg / L 6-benzylpurine, 0.6 mg / L zeatin, 2.0 g / L gibberellin, 2.0 mg / L ammonia water, 40 g / L glucose and 4.0 g / L agar, adjust The pH value of the induction medium was 5.8-6.2; the culture conditions were as follows: the culture temperature was 27 °C, the light intensity was 2500 Lux, and the light time was 11 h / d.

[0030] (2) Place the callus of the top buds of Be...

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PUM

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Abstract

The invention discloses a rapid propagation method for malus halliana. The method comprises steps of primary culture, subculture, rooting culture and plantlet hardening and transplanting. The malus halliana culture with a provided culture medium has the advantages of low planting cost, low investment risks, high survival rate after transplanting, strong plant vigor, short culture period and high technical practicability, is suitable for large-area popularization and planting, a good foundation is laid for large-scale artificial planting of the malus halliana and strengthening of product competitiveness, and the method has good economic benefit and social benefit.

Description

technical field [0001] The invention belongs to the technical field of planting and cultivation, and in particular relates to a method for rapid propagation of crabapple. Background technique [0002] Chuisi Begonia is a deciduous tree belonging to the Rosaceae apple genus. It is an important ornamental tree species. It is cultivated in various places. Its taste is light, bitter, and flat in nature. Its flowers are used as medicine. It has the effect of regulating menstruation and activating blood circulation. For bruises, fractures and other diseases, the dying crabapple also has certain edible functions. Its fruit is sweet and sour, and can be made into candied fruit. It is used in a large amount in food and health products, especially in recent years. The demand for Chuisi Begonia is also increasing. Usually, the methods of breeding Chuisi Begonia mainly include ramets, grafting, layering, and cuttings. The ramets germinate from the rhizosphere of the mother plant in Marc...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01G24/25A01G24/27A01G24/22A01G24/15
CPCA01G31/00A01H4/001A01H4/008A01G24/15A01G24/22A01G24/25A01G24/27
Inventor 丁福冬
Owner 句容市茂润苗木有限公司
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