Cell morphology reconstruction oriented three-dimensional image information acquisition method and device

Abstract

The invention discloses a cell morphology reconstruction oriented three-dimensional image information acquisition method and device. An objective table is fixedly connected with four vertically arranged micro telescopic rods; screws at the upper ends of the micro telescopic rods are fixedly connected to four corners of a glass slide rack in sequence; motors in the micro telescopic rods are connected with a motor control module by virtue of control lines; the motor control module is connected with an upper computer by virtue of an MCU (Micro Control Unit) system 23; a camera is positioned justabove the motor control module; an optical collector is arranged outside the camera in a sleeving manner and is composed of a halogen lamp, an organic panel, the micro telescopic rods and micro telescopic rod fixing bases; the motors in the micro telescopic rods are connected with the motor control module by virtue of control lines; and the motor control module is connected with an upper computerby virtue of the MCU system. The inclination of the cell slide is changed by regulating the heights of two adjacent micro telescopic rods on the objective table, the small-angle rotation of the slideand rotation surface direction change of the slide are realized, and irrelevant three-dimensional morphology information is acquired, so that the morphology reconstruction accuracy is improved.

Description

technical field [0001] The invention relates to a technique for analyzing cell shape characteristics, in particular to a method and device for acquiring three-dimensional image information for cell shape reconstruction. Background technique [0002] The analysis of cell morphological characteristics has always been the most important method for studying biological cytology and pathology, and it is widely used in the fields of medical medicine, clinical diagnosis, health care industry and disease prevention. The acquisition of 3D image information of cell morphology has a direct impact on the reconstruction results. At present, the commonly used methods for acquiring 3D image information of cells are mainly as follows: 1. Optical acquisition of 3D information by lens array: the principle is to transform the image recognition of 2D objects into the recognition of 3D objects, but for objects that rotate at a small angle It is not easy to realize, and this method requires speci...

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Problems solved by technology

At present, the commonly used methods for acquiring 3D image information of cells are mainly as follows: 1. Optical acquisition of 3D information by lens array: the principle is to transform the image recognition of 2D objects into the recognition of 3D objects, but for objects that rotate at a small angle It is not easy to realize, and this method requires special equipment, and the cost is too high; 2. Three-dimensional display of the lens plate: this method has the advantages of high electro-optical transmittance and electronic control adjustable, but its effective refractive index is low, the response speed is slow, and the resolution The rate is low and the reconstruction accuracy is not high

The Chinese Patent Publication No. CN105184853A, titled "A Single-Cell Three-dimensional Image Generation Method Based on Optical Flow Analysis" discloses that a non-uniform electric field is generated on the light-induced dielectrophoresis chip produced by incident light irradiation, and then the input is changed. The frequency and magnitude of the electrode AC signal are used to control the magnitude and direction of the dielectrophoretic force on the cell, so as to achieve the purpose of obtaining the three-dimensional image shape of the cell rotation, but this method has the following disadvantages: First, during the cell rotation process, Due to the greater influence of fluid motion, it is difficult to achieve directional rotation of cells; second, it is difficult to achieve precise rotation during the process of acquiring its morphological images, which may easily cause image information correlation between similar surfaces

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