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Good-effect mercury chelating type immune complex and preparation method thereof

An immune complex and chelating technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem that the preparation method of mercury chelating immune complex is not perfect, prolongs the preparation cycle of the target object, and does not care about the wide range of applications, etc. problems, to achieve the effects of shortening the preparation cycle, large market competitiveness, and wide application range

Inactive Publication Date: 2018-11-06
广东腾湃医疗股份有限公司
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Problems solved by technology

[0002] Chelates are widely used in mineral flotation process, hydrometallurgy, extraction and separation of metal elements, catalytic synthesis of substances, water softening, electroplating process, pharmaceutical industry, dyeing process, etc.; the existing mercury chelate The preparation method of the compatible immune complex is not perfect, and the scope of application is not wide enough. It is inconvenient to complete the preparation process of the target in a small laboratory, and the number of dialysis will prolong the preparation period of the target to a certain extent. There is no clear treatment of ions and other wastes in the waste liquid, and the discharge of ions and other wastes in the waste liquid will cause chemical pollution if they are not treated up to the standard

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  • Good-effect mercury chelating type immune complex and preparation method thereof

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preparation example Construction

[0033] A kind of preparation method of mercury chelated immune complex as claimed in claim 1, comprising the following steps:

[0034] S1: The chelating agent solution is prepared first, and then the chelating agent is dissolved in the solvent to obtain the chelating agent solution;

[0035] S2: take an appropriate amount of carrier protein, add the carrier protein to the borate buffer solution, and then prepare the carrier protein solution;

[0036] S3: adding the prepared chelating agent solution into the carrier protein solution, placing it in a constant temperature water bath and stirring evenly, and then in a constant temperature water bath for 24 hours to obtain a mixed solution;

[0037] S4: Take the dialysis bag and add EDTA-NaHCO to the dialysis bag 3 The solution is boiled, the dialysis bag is fixed in the middle of the tank, and sufficient ddH is added to the tank 2 O, the ddH 2 The volume ratio of the volume of O to the solution in the dialysis bag is 83:1, repe...

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Abstract

The invention discloses a good-effect mercury chelating type immune complex. The good-effect mercury chelating type immune complex is one of the following complexes: a complex formed by binding mercury ions with an immune complex, a complex formed by binding the mercury ions with an antibody which is in specific binding with carrier protein after binding the mercury ions with the carrier protein or a complex formed by binding the mercury ions with the carrier protein after binding the mercury ions with immune globulin. The invention also discloses a preparation method of the good-effect mercury chelating type immune complex. The preparation method comprises the following steps: S1, preparing a chelating agent solution; S2, preparing a carrier protein solution; S3, stirring and staying overnight; S4, carrying out dialysis treatment; S5, adding the mercury ions; S6, carrying out recovery treatment on waste liquid; S7, carrying out specific binding. The preparation method disclosed by theinvention is wider in application range, the cost can be saved, the dialysis rate is increased, a preparation period can be shortened, the preparation method also has the characteristics of energy conservation and environment protection, chemical pollution can be prevented from being caused, and an environment protection effect is good.

Description

technical field [0001] The invention relates to the field of chelate compounds, in particular to a mercury chelate immune complex with excellent effect and a preparation method thereof. Background technique [0002] Chelates are widely used in mineral flotation process, hydrometallurgy, extraction and separation of metal elements, catalytic synthesis of substances, softening of water, electroplating process, pharmaceutical industry, dyeing process, etc. The preparation method of the combined immune complex is not perfect, the scope of application is not wide enough, it is inconvenient to complete the preparation process of the target in a small laboratory, and the number of dialysis is too many, which will prolong the preparation cycle of the target to a certain extent. There is no clear treatment of ions and other wastes in the waste liquid. If the ions and other wastes in the waste liquid are not treated up to the standard, they will cause chemical pollution. SUMMARY OF ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 张积仁陈振展傅世林常承瑜李波
Owner 广东腾湃医疗股份有限公司
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