Biomarkers for the detection of idiopathic inflammatory myopathy and their use
A technology of uses and biological samples, applied in the field of biomarkers for the detection of polymyositis and/or dermatomyositis, which can solve problems such as difficulty in early diagnosis, early treatment, misdiagnosis of patients, and complex clinical manifestations
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Embodiment 1
[0028] Example 1 High-density protein chip screening PM / DM related autoantibodies
[0029] The N-terminal of all protein antigens on the high-density protein chip has a GST tag for protein purification. First, use rabbit anti-GST monoclonal antibody to hybridize with chip protein to verify chip quality. Then, 40 PM / DM patient sera, 30 autoimmune disease control sera, and 20 healthy control sera were selected for hybridization with 90 high-density protein chips, and candidate PM / DM-related autoantigens were identified through signal acquisition and data analysis.
[0030] 1.1 Extraction of high-density protein chip scanning data
[0031] The GenePix 4000B chip scanner was used to scan the high-density protein chip, and after obtaining the grayscale image in TIFF format, according to the operation manual of the GenePix Pro 6.0 software, and according to the high-density protein chip loading matrix parameters, the analysis software automatically completed each protein point. Se...
Embodiment 2
[0052] Example 2 Construction of PM / DM Related Autoantibody Target Antigen Protein Chip and Serum Screening Verification
[0053] Due to differences in genetic background and environmental factors in patients with autoimmune diseases, the types of autoantibodies produced by different patients with the same autoimmune disease are quite different. In order to confirm whether the PM / DM-related autoantibodies screened from small samples by high-density protein chips have clinical significance and application value, as well as the sensitivity and specificity of these autoantibodies to the diagnosis of PM / DM patients, this study will use Example 1 PM / DM-related autoantibody target antigens screened by high-density protein chips were prepared into PM / DM-related autoantibody target antigen protein chips for clinical expansion sample verification. Serum samples used for PM / DM-related autoantibody target antigen protein chips for clinical large-scale validation testing included 397 PM / D...
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