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Forensic science composite detection kit based on Y-snp genetic markers of 20 haplogroup D

A Y-SNP and genetic marker technology, applied in the field of forensic composite detection kits, can solve the problem of inability to be assigned to haplogroup D twigs, etc., achieve good group specificity and geographic specificity, accurate and reliable typing results, The effect of widespread application and promotion of value

Active Publication Date: 2021-01-26
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: the existing detection kit can only accurately assign the sample to the Y chromosome, but cannot more accurately assign it to the twig of haplogroup D

Method used

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  • Forensic science composite detection kit based on Y-snp genetic markers of 20 haplogroup D
  • Forensic science composite detection kit based on Y-snp genetic markers of 20 haplogroup D
  • Forensic science composite detection kit based on Y-snp genetic markers of 20 haplogroup D

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] The preparation of embodiment 1 kit of the present invention

[0083] The SNP genetic marker composite detection kit for 20 Y chromosome haplogroup D for detection may include the following reagents packaged separately:

[0084] a) Multiplex amplification primer mix. It was obtained by mixing the amplification primers shown in Table 2. The amplification primers were synthesized by Invitrogen Company. The synthetic amplification primers were prepared with ultrapure water to 100pM / μL, and then mixed according to the ratio in Table 6 to make a composite amplification primer. Primer mix.

[0085] b) Compound amplification reaction mixture. The PCR reaction mixture MultiplexPCRMix of Qiagen Company was used in this embodiment.

[0086] c) Multiple single base extension reaction primer mix. It was obtained by mixing the single-base extension reaction primers shown in Table 3, and the single-base extension reaction primers were all synthesized by Invitrogen Company. The s...

Embodiment 2

[0095] Example 2 Using the kit of the present invention to detect 200 unrelated individual samples roughly classified into haplogroup D

[0096] Using the above-mentioned forensic science composite detection kit based on 20 Y-SNP genetic markers of haplogroup D, 200 samples of unrelated individuals roughly classified as haplogroup D were tested. The specific detection process is as follows:

[0097] a. The DNA of 200 samples was extracted by the Chelex-100 method as a composite amplification template;

[0098] b. Using the DNA in step a as a template, use the composite amplification primer mixture and the composite amplification reaction mixture to perform composite PCR amplification on the sample in the following amplification system; 0.3 μL of the composite amplification primer mixture, the composite amplification reaction Mixture 2.5 μL, template DNA 0.8 μL, add ddH2O to 5 μL; PCR amplification conditions: 95°C, 15 minutes; 94°C, 30 seconds; 66°C-60°C, 90 seconds; 72°C, 30...

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Abstract

The invention belongs to the field of forensic medicine detection kits, in particular to a forensic medicine composite detection kit based on 20 Y-SNP genetic markers of haplogroup D. Aiming at the problem that the existing detection kit can only accurately assign the sample to the Y chromosome, but cannot more accurately assign it to the twig of haplogroup D, the present invention provides a composite detection kit, comprising: separating Packaged Multiplex Amplification Primer Mix, Multiplex Single Base Extension Reaction Primer Mix and Standard DNA 2800M. The kit of the present invention applies multiple amplification and single base extension technology, and can obtain the genotyping of 20 SNP genetic markers of Y chromosome haplogroup D from biological samples at one time, and will belong to haplogroup D in China. The male samples are accurately divided into the twigs of the haplogroup D of the high-resolution Y chromosome phylogenetic tree, and the family origin can be quickly inferred.

Description

technical field [0001] The invention belongs to the field of forensic medicine detection kits, in particular to a forensic medicine composite detection kit based on 20 Y-SNP genetic markers of haplogroup D. Background technique [0002] The Y chromosome is a chromosome unique to males. The total length of the Y chromosome DNA is about 60Mb, and the two ends account for about 5% of the Y chromosome, which is the pseudoautosomal region (PAR). During the meiosis process, the pseudoautosomal region can recombine with the corresponding segment of the X chromosome. For exchange, the remaining 95% of the region is non-recombiningY (NRY), which will not recombine with any chromosomal segment. The Y chromosome can only be passed from father to son. In the case of ignoring mutations, the offspring of the same family have exactly the same non-recombination region of Y chromosome. Therefore, the Y chromosome not only determines the sex, but also has genetic characteristics different f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858
CPCC12Q1/6858C12Q2531/113C12Q2537/143C12Q2533/101C12Q2565/125
Inventor 侯一平李介男叶懿宋凤罗海玻谢明坤郎敏王正钱小钦李英碧
Owner SICHUAN UNIV
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