Method suitable for liquid culture of grape micro-grafted seedlings

Abstract

The invention relates to a method suitable for liquid culture of grape micro-grafted seedlings. The method is characterized by comprising the following steps of (1) using rootstock variety tissue culture seedlings cultivated for 30-40 days as a material; (2) using scion variety tissue culture seedlings cultivated for 30-40 days as a material; (3) inserting oblique surface ends of scions into the incisions of rootstocks under sterile conditions to form grafted seedlings; (4) pressing two ends of a U-shaped clip with tweezers to make the U-shaped clip clamp and fix grafting interfaces of the grafted seedlings; (5) inserting the grafted micro-grafted seedlings into prepared loofah, and then placing the loofah with the micro-grafted seedlings into a culture flask containing 50 ml of liquid media for cultivation. The method has the advantages that (1) a liquid medium formula suitable for combination culture of a plurality of grape rootstocks and scions is screened, the rooting rate of the grape grafted seedlings cultured by the media is 100%, and the roots cultured are more developed than solid culture.

Description

technical field [0001] The invention relates to a method suitable for liquid culture of grape micro-grafted seedlings. Background technique [0002] Micro-grafting technology is a technique that combines tissue culture and grafting, and grafts rootstocks and scion tissue culture seedlings of different varieties under tissue culture conditions. Since the 1950s when Dollinger Bosch used ivy to first apply stem tip grafting technology, micro-grafting technology has developed rapidly. The United States, France, Italy, Spain, South Africa, Israel, Japan and China have successively applied it in the micro-grafting of citrus, apple, peach, cherry, apricot, plum, strawberry, grape, avocado, almond apricot, jujube, Korla pear and other fruit trees. . This technology is now mainly used for the rapid breeding of fruit tree grafted seedlings, the cultivation of virus-free seedlings, the identification of grafting affinity, the detection of viruses, and the research on the mechanism of...

AI Technical Summary

Problems solved by technology

Although very few studies have carried out the liquid culture of micro-grafted seedlings of fruit trees, the supports of the liquid culture are mainly paper bridges, and the grafted seedlings tend to fail due to damage to the paper bridges during the cultivation process.

In China, there have been relevant research reports on the cultivation of grape micro-grafting seedlings, but the grafting survival rate is low (less than 70%), and there are no relevant reports on grape micro-grafting liquid culture

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