TT7 kit based on micro-fluidic chip for detecting total thyroxine and preparation and detection methods

Abstract

The invention discloses a TT7 kit based on a micro-fluidic chip for detecting total thyroxine and preparation and detection methods. The preparation method of the kit comprises the following steps: (1) coating the micro-fluidic chip; (2) marking fluorescent microspheres; (3) assembling the micro-fluidic chip; (4) preparing a calibrated product; and (5) obtaining the total thyroxine TT4 kit based on the micro-fluidic chip. The prepared total thyroxine TT4 kit is determined by adopting a competition law. By selecting a highly sensitive time resolution phosphor as a marker and marking the antigenon the fluorescent microspheres and carrying out analysis and detection by means of an immune competitive reaction, the performance of the prepared kit can reach the same chemiluminiscent reagent level. After immune reaction, residual components are fully removed by using a cleaning liquid, and the reading on the fluorescent microspheres bonded to the immune reaction is detected, so that the problem that a developing liquid which is introduced is insufficient in reaction after entering the chip or the reading is not immediate after developing is solved.

Description

technical field [0001] The invention belongs to the field of immune detection, and in particular relates to a microfluidic chip-based detection kit for total thyroxine TT4 and a preparation and detection method. Background technique [0002] Microfluidic chip technology is characterized by a micropipeline network, using micromachining technology to etch a micropipeline network and other functional units on a chip of a few square centimeters in size, thereby preparing a microfluidic chip that includes injection, reaction, separation, and detection An integrated fast, efficient, and low-consumption micro-analysis device. The microfluidic chip has the characteristics of less reagent consumption, short reaction time and high degree of automation in the detection platform. In the current research, the miniaturization of the chip has brought many difficulties to the early sample separation and supporting integration, and there are also many problems in sample fixation and elution...

AI Technical Summary

Problems solved by technology

[0013] Using other existing technologies or (1) the structure of the chip is too complicated to precisely regulate the reaction, and there is a large pollution in the cleaning steps and liquid conduction; or (2) multiple liquid chambers and solid phase carriers are used (magnetic beads and fluorescent microspheres are used at the same time), the overall design of the reagent is too complicated; or (3) the immunoreaction adopts a heterogeneous reaction, which means that the solid phase carrier reacts with the surface of the solid phase carrier, the reaction is insufficient, and the sensitivity and Repeatability is limited

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