Thyroxine chemiluminescence immune analysis quantitative measuring reagent kit and method for preparing the same
A chemiluminescence and quantitative determination technology, applied in the field of immunodiagnosis, can solve the problems of high price, complicated operation, and difficult promotion
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[0069] 1. Preparation of chemiluminescence substrate solution:
[0070] Solution A: Add 1.21g Tris and 295μL concentrated HCl to 100ml double distilled water to make 0.1M Tris-HCl buffer solution with pH8.5. Add 0.5 g of Luminol, 20 μL of Tween20, and 0.1 g of benzofluoranthene (the structure is shown in the figure below) to this buffer, and mix well.
[0071] Solution B: Add 0.73 g of trisodium citrate and 0.44 g of citric acid to 100 ml of double distilled water to prepare a citric acid buffer solution of 0.1M pH4.6, and add 100 μL of 30% hydrogen peroxide solution to this solution.
[0072] How to use: Mix liquid A and liquid B at a ratio of 1:1 before use.
[0073]
[0074] 2. Preparation of enzyme markers:
[0075] 2.1 Biotinylated T4
[0076] (1) Connection between T4 and BSA
[0077] Take 1 mg of T4 whose carboxyl group has been methylated and dissolve it in 0.2 mL DMF to form a 5 mg / mL solution, add 1 mL of BSA with a concentration of 0.5 mg / mL (dissolved in 0.0...
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