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Medicine for artificially inducing rockfish's sex reversion and its preparation

A grouper sex, artificial induction technology, applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc. The effect of shortened sexual transition time and advanced high-tech content

Inactive Publication Date: 2004-09-22
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the late 1990s, the folks in Taiwan improved the broodstock breeding system and used cages to breed natural female and male broodstock to fertilize eggs naturally, and sold the fertilized eggs or hatched fry to the coastal areas of Guangdong, but the hatching rate of fertilized eggs was less than 1%. ; Due to the limited number of male broodstock, the number of fry is extremely limited, and the price is expensive
This approach cannot fundamentally solve the industrialization of grouper artificial breeding and the demand for large-scale breeding of seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] When using 23% of methyltestosterone (17αMethyltestosterone, 17αMT), 56% of testosterone, 16% of testosterone propionate and 5% of thyroxine, add 8% seaweed polysaccharide and mix well , with sterile seawater bony fish physiological saline to make a sticky mass, squeeze it hard to make a cylindrical drug strip, the broodstock used in the experiment was anesthetized with 0.5% urethane, and the implanted drug strip was located behind the upper edge of the operculum of the head 14cm, transdermal puncture into the back muscle at 4cm on both sides of the midline of the back; the base of the wound is anti-inflammatory with an appropriate amount of egg white glycerin mixed with 1% thymol and 0.01% nitrofurazone, and then use a double-layer sand filter for seawater immersion experiments Broodstock, after waking up, slowly move into the intensive breeding pond. After the above operation, the parent fish implanted with the mixed hormone drug strip not only survived 100%, but also...

Embodiment 2

[0025] When using 28% of methyl testosterone (17αMethyltestosterone, 17αMT), 40% of testosterone, 17% of testosterone propionate and 15% of thyroxine, add 18% of α-starch and mix well Evenly, use sterile seawater bony fish saline to make a sticky mass, squeeze it hard to make a cylindrical drug strip, and use MS222 to anesthetize the broodstock in the experiment. The implanted drug strip is located 12cm behind the upper edge of the operculum of the head, and deviates from the middle of the back. puncture into the back muscle at 4cm on both sides of the line; the base of the wound is anti-inflammatory with an appropriate amount of egg white glycerin mixed with 1% thymol and 0.01% nitrofurazone, and then use a double-layer sand filter to immerse the broodstock in seawater until it wakes up Then slowly move into the intensive cultivation pool. After the above operation, the parent fish implanted with the mixed hormone drug strip not only survived 100%, but also realized the sex r...

Embodiment 3

[0027] When using 27% of methyltestosterone (17αMethyltestosterone, 17αMT), 49% of testosterone, 11% of testosterone propionate and 13% of thyroxine, add 6% seaweed polysaccharide and mix well , with sterile seawater bony fish physiological saline to make a sticky mass, squeeze it hard to make a cylindrical drug strip, the broodstock used in the experiment was anesthetized with 0.5% urethane, and the implanted drug strip was located behind the upper edge of the operculum of the head 10cm, transdermal puncture into the back muscles at 3.8cm on both sides of the midline of the back; the base of the wound was anti-inflammatory with an appropriate amount of egg white glycerin mixed with 1% thymol and 0.01% nitrofurazone, and then used a double-layer sand filter for seawater immersion experiments Use broodstock, and slowly move into the intensive breeding pond after waking up. After the above operation, the parent fish implanted with the mixed hormone drug strip not only survived 1...

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PUM

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Abstract

The medicine has mixed hormone comprising methyl testosterone, testosterone, propiotestosterone and thyroxine, and the mixture is added with algal polysaccharides or alpha-starch and prepared into mediicne stip suitable for embedded into fish body. The medicine of the present invention is embedded into female rockfish's body to induce sexual reversion. Practical test shows that using the mixed external hormone of the medicine can shorten female rockfish's sexual reversion period to 80 days with stable and reliable effect and no sexual restoring.

Description

(1) Technical field [0001] The invention relates to a fish denaturation medicine in aquaculture and its preparation. (2) Background technology [0002] Grouper belongs to Serranidae, Epinephelinae, and Epinephelus. There are more than 400 species in the world, and 45 species have been recorded in the coastal areas of my country's mainland (Zhu Qian, 1998). The sexual transformation process of "female first and then male" is common in the species, and the male broodstocks are all aging (generally over 6 years old); in large species (such as: giant grouper), although the females are mature and can ovulate by the 4th age; It turns into a functional male fish when it is over 10 years old and weighs more than 30 kilograms. The sexual reversal process of more than 6 years not only consumes a lot of fish bait, but also the existence of intersex bodies delays the time of artificial reproduction; when the growth and development of the male testis can release sperm, the population is v...

Claims

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Application Information

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IPC IPC(8): A61K38/22A61P15/08
Inventor 邹记兴
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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