Method for removing thyroxine and triiodothyronine from human serum

A technology for triiodothyronine and thyroxine, applied in the field of serum, can solve the problems of complicated process, excessive material loss and production cost, and achieve the effects of simple operation, low production cost and high clearance rate

Inactive Publication Date: 2016-06-08
GUANGZHOU KEFEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing dethyroxine and triiodothyronine human serum preparation process, due to many reasons such as complex process and excessive material loss, the production cost is too high

Method used

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  • Method for removing thyroxine and triiodothyronine from human serum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for removing thyroxine and triiodothyronine in human serum, comprising the steps of:

[0023] (1) Take human serum, centrifuge at 12000g for 0.2h, and take the supernatant;

[0024] (2) Add 0.05% sodium azide of human serum weight and activated carbon of 8% human serum weight, stir gently at room temperature for 5 hours, centrifuge at 12000g for 0.5 hours, and remove the precipitate;

[0025] (3) Add kaolin with 10% weight of human serum, stir for 15 hours and then centrifuge at 12000g for 0.5 hours to obtain supernatant.

Embodiment 2

[0027] A method for removing thyroxine and triiodothyronine in human serum, comprising the steps of:

[0028] (1) Take the isolated human serum, centrifuge at 12000g for 0.2h, and take the supernatant;

[0029] (2) Add 0.05% human serum weight sodium azide and 8% human serum weight activated carbon with a particle size of 220 mesh, stir gently at room temperature for 5 hours, then centrifuge at 12000g for 0.5 hour to remove the precipitate;

[0030] (3) Add 10% human serum weight kaolin with a particle size of 200 mesh, stir for 15 hours and centrifuge at 12000g for 0.5 hours to obtain supernatant.

[0031] (4) Filter the supernatant obtained in step (3) through 0.8 μm, 0.45 μm and 0.22 μm filter membranes in sequence, and harvest the filtrate.

Embodiment 3

[0033] A method for removing thyroxine and triiodothyronine in human serum, characterized in that it comprises the steps:

[0034] (1) Take human serum, centrifuge at 16000g for 1.0h, and take the supernatant;

[0035] (2) Add 0.08% human serum weight sodium azide and 15% human serum weight activated carbon with a particle size of 180 mesh, stir gently at room temperature for 16 hours, then centrifuge at 16000g for 2 hours to remove the precipitate;

[0036] (3) Add 20% human serum weight kaolin with a particle size of 150 mesh, stir for 30 hours and then centrifuge at 16000 g for 2 hours to obtain the supernatant.

[0037] (4) Filter the supernatant obtained in step (3) through 0.8 μm, 0.45 μm and 0.22 μm filter membranes in sequence, and harvest the filtrate.

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Abstract

A method for removing thyroxine and triiodothyronine from human serum is characterized by comprising steps as follows: adding activated carbon to human serum, performing stirring, and removing activated carbon; then adding kaolin, performing stirring, and removing kaolin. The method for removing thyroxine and triiodothyronine from the human serum has the advantages that the operation is simple, the production cost is low, the removal rate of thyroxine and triiodothyronine is high, and the like.

Description

technical field [0001] The invention relates to the field of serum, in particular to a method for removing thyroxine and triiodothyronine in human serum. Background technique [0002] Thyroxine (T4) and triiodothyronine (T3) are two iodine-containing hormones secreted by the thyroid gland, which are responsible for regulating the metabolism of substances and energy in the human body, and promoting the growth and development of the human body. In normal human blood, T3 and T4 exist in free form and protein-bound form, and the two forms are in dynamic balance. More than 99.6% of total T3 and total T4 in blood exist in protein-bound form, and only 0.2%-0.4% of total T3 and total T4 exist in blood as unbound or free form. [0003] At present, in the in vitro diagnostic kits at home and abroad, most reagent manufacturers use dethyroxine and triiodothyronine human serum as the matrix due to the stability and matrix effect of the standard and quality control products prepared by T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34
CPCG01N1/34G01N2001/2893
Inventor 陆建斌
Owner GUANGZHOU KEFEN BIOTECH CO LTD
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