Establishment and application of animal model for ulcerative colitis with liver stagnation and spleen deficiency syndromes

A technology for ulcerative colitis and animal models, applied in the field of traditional Chinese medicine, can solve the problems of short duration of the disease course and the inability of the model to clarify the pathogenesis.

Active Publication Date: 2018-12-11
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most studies on ulcerative colitis use chemical stimulation to prepare animal models of UC, but the duration of the disease is relatively short, and there are still many models that cannot clarify its pathogenesis and other shortcomings

Method used

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  • Establishment and application of animal model for ulcerative colitis with liver stagnation and spleen deficiency syndromes
  • Establishment and application of animal model for ulcerative colitis with liver stagnation and spleen deficiency syndromes
  • Establishment and application of animal model for ulcerative colitis with liver stagnation and spleen deficiency syndromes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Chronic restraint-induced liver depression and spleen deficiency syndrome ulcerative colitis animal model establishment method

[0025] 1.1 Experimental reagents and instruments

[0026] 1.1.1 Animals: 16 healthy SD rats, male, SPF level, body weight (210±10) g, purchased from Kunming Chushang Technology Co., Ltd., animal certification number SYXK (Dian) K2012-0002, experimental animals of Dali University Central IVC Animal House.

[0027] Feeding, drinking water freely, feeding 12g / bird / day, in a room with constant temperature (20±2)°C and humidity (50±2)%, light 12h day / 12h night, and adaptive feeding for 1 week before carrying out the experiment.

[0028] 1.1.2 Reagent: 2,4,6-trinitrobenzene-sulfonic acid (Trinitro-benzene-sulfonic acid, TNBS), batch number: SLBP08899V, American Sigma Company; absolute ethanol (AR), batch number: 20140523, Tianjin Fengchuan Chemical Reagent Technology Co., Ltd.; formaldehyde solution (AR), lot number: 20140523, disodium h...

Embodiment 2

[0032] Example 2 Chronic restraint, overwork and diet indiscipline to induce liver depression and spleen deficiency ulcerative colitis animal model establishment method

[0033] 2.1 Experimental reagents and instruments

[0034] 2.1.1 Animals: 25 healthy SD rats, male, SPF level, body weight (210±10) g, purchased from Kunming Chushang Technology Co., Ltd., animal certificate number SYXK (Dian) K2012-0002, experimental animals of Dali University Central IVC Animal House.

[0035] Feeding, drinking water freely, feeding 12g / bird / day, in a room with constant temperature (20±2)°C and humidity (50±2)%, light 12h day / 12h night, and adaptive feeding for 1 week before carrying out the experiment.

[0036] 2.1.2 Reagent: 2,4,6-trinitrobenzene-sulfonic acid (Trinitro-benzene-sulfonic acid, TNBS), batch number: SLBP08899V, American Sigma Company; absolute ethanol (AR), batch number: 20140523, Tianjin Windboat Chemical Reagent Technology Co., Ltd.; formaldehyde solution (AR), lot number...

Embodiment 3

[0039] Example 3 Method of establishing an animal model of ulcerative colitis with liver depression and spleen deficiency induced by tail pinch, overwork and diet

[0040] 3.1 Experimental reagents and instruments

[0041] 3.1.1 Animals: 16 healthy SD rats, male, SPF level, body weight (210±10) g, purchased from Kunming Chushang Technology Co., Ltd., animal certificate number SYXK (Dian) K2012-0002, experimental animals of Dali University Central IVC Animal House.

[0042] Feeding, drinking water freely, feeding 12g / bird / day, in a room with constant temperature (20±2)°C and humidity (50±2)%, light 12h day / 12h night, and adaptive feeding for 1 week before carrying out the experiment.

[0043] 3.1.2 Reagent: 2,4,6-trinitrobenzene-sulfonic acid (Trinitro-benzene-sulfonic acid, TNBS), batch number: SLBP08899V, American Sigma Company; absolute ethanol (AR), batch number: 20140523, Tianjin Fengchuan Chemical Reagent Technology Co., Ltd.; formaldehyde solution (AR), lot number: 201...

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Abstract

The invention provides a method for the establishment of an animal model for ulcerative colitis with liver stagnation and spleen deficiency syndromes. The method comprises the following steps of enabling rats to swim previously before modeling, removing unqualified rats, enabling the qualified rats to be subjected to chronic restraint, overwork and eating disorder treatment, molding for 7 days, performing clysis on the 8th day to establish an ulcerative colitis model, forbidding the rats from fasting and drinking water for 24 hours, anesthetizing through intraperitoneal injection, then slowlyinjecting a TNBS/ethanol solution from the anus of the rats, keeping the rats in a full-flat state after enabling the rats to stand on the head, putting the rats in a clean rat frame, enabling the rats to wake naturally, and enabling the rats to continue to be subjected to chronic restraint, overwork and eating disorder treatment from the 9th day so as to obtain the animal model for the ulcerativecolitis with the liver stagnation and spleen deficiency syndromes on the 28th day. According to the method, the repeatability is good, the operation is simple and easy, a UC model which more conformsto the combination of traditional Chinese medicine theory with modern medicine is obtained, the model can be used for studying the occurrence and development of UC diseases, a reliable model for screening medicine for treating the UC diseases is provided, and the model is of great significance for the treatment of the UC diseases.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to the establishment and application of an animal model of ulcerative colitis with liver depression and spleen deficiency syndrome. Background technique [0002] Ulcerative colitis (Ulcerative colitis, UC) is a kind of intestinal chronic non-specific colitis whose etiology is not yet fully clarified. Colonic mucosa and submucosa are involved, mostly starting from the distal colon, and can develop retrogradely to the proximal segment, even involving the entire colon, and occasionally involving the terminal ileum. With the global changes in society and economy, people's lifestyles, eating habits and environment have undergone tremendous changes, which has led to changes in the spectrum of diseases. The increase in the incidence of UC ulcers year by year is the epitome of this change. Based on the results of many domestic hospitals According to case statistics, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/185A61K36/71A61K36/65A61P1/04A61P1/00A61K31/635
CPCA61K31/185A61K31/635A61K36/076A61K36/232A61K36/233A61K36/284A61K36/484A61K36/534A61K36/65A61K36/71A61P1/00A61P1/04A61K2300/00
Inventor 刘衡何苗孙敏哲余万鑫李玥张枝雪唐苗赵昱
Owner DALI UNIV
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